Whole genome amplification articles within Nature Communications

Featured

• Article
  15 February 2019 | Open Access

  Single gametophyte sequencing reveals that crossover events differ between sexes in maize

  Meiotic crossover (CO) landscape differs inter- and intra-species, as well as between sexes. Here, the authors show that male meiosis produces more COs than female in maize and detect CO maturation inefficiency in some genetic backgrounds, which may help to improve breeding efficiency.

  • Cheng Luo
  • , Xiang Li
  •  & Jianbing Yan

• Article
  29 November 2016 | Open Access

  TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol

  Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and demonstrate its utility and accuracy.

  • Ángel J. Picher
  • , Bettina Budeus
  •  & Armin Schneider

• Article | 16 April 2015

  Calibrating genomic and allelic coverage bias in single-cell sequencing

  Artifacts caused by whole-genome amplification bias are a recurrent challenge in single-cell sequencing analysis. Here, the authors develop statistical models and demonstrate an efficient strategy for controlling amplification errors by a joint analysis of single cell genomes.

  • Cheng-Zhong Zhang
  • , Viktor A. Adalsteinsson
  •  & J. Christopher Love

• Article
  06 March 2015 | Open Access

  Whole-genome sequence-based analysis of thyroid function

  Levels of circulating thyrotropin and free thyroxine reflect thyroid function, however, their genetic underpinnings remain poorly understood. Taylor et al. take advantage of whole-genome sequence data from cohorts within the UK10K project to identify novel variants associated with these traits.

  • Peter N. Taylor
  • , Eleonora Porcu
  •  & Pingbo Zhang