Western blot articles from across Nature Portfolio

The western blot is a method for detecting the presence and size of specific proteins. Proteins are first separated according to size or other physical properties by gel electrophoresis, then transferred to a membrane (blotted) before a detection step that uses labelled antibody to recognize the protein of interest.

Latest Research and Reviews

Research
21 July 2022 | Open Access

Beovu, but not Lucentis impairs the function of the barrier formed by retinal endothelial cells in vitro
- Heidrun L. Deissler
- , Catharina Busch
- & Matus Rehak
Scientific Reports 12, 12493
Research
30 May 2019 | Open Access

Epidermal Growth Factor Stimulates Transforming Growth Factor-Beta Receptor Type II Expression In Corneal Epithelial Cells
- Daisy Y. Shu
- , Audrey E. K. Hutcheon
- & Xiaoqing Guo
Scientific Reports 9, 8079
Research
25 February 2019 | Open Access

Dengue virus nonstructural 3 protein interacts directly with human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and reduces its glycolytic activity
- Emiliana M. Silva
- , Jonas N. Conde
- & Ronaldo Mohana-Borges
Scientific Reports 9, 2651
Research
11 September 2017 | Open Access

Molecular details of secretory phospholipase A₂ from flax (Linum usitatissimum L.) provide insight into its structure and function
- Payal Gupta
- & Prasanta K. Dash
Scientific Reports 7, 11080
Research
26 September 2016 | Open Access

Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations
- Jessica S. Sadick
- , Molly E. Boutin
- & Eric M. Darling
Scientific Reports 6, 33999
Protocols | 28 July 2016

Single cell–resolution western blotting

Single-cell-resolution western blotting concatenates an electrophoretic separation to downstream immunoprobing of unfixed mammalian cells to detect proteins, isoforms, and interactions that are not discernable by immunoreactivity alone.
- Chi-Chih Kang
- , Kevin A Yamauchi
- & Amy E Herr
Nature Protocols 11, 1508-1530

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News and Comment

Research Highlights | 30 November 2018

Enhanced antibody validation
- Allison Doerr
Nature Methods 15, 1001
Correspondence | 28 February 2017

Should we ignore western blots when selecting antibodies for other applications?
- Fridtjof Lund-Johansen
- & Michael D Browning
Nature Methods 14, 215
Correspondence | 28 February 2017

Response to: Should we ignore western blots when selecting antibodies for other applications?
- Mathias Uhlen
Nature Methods 14, 215-216
Research Highlights | 23 June 2014

Single-cell western blotting
- Eytan Zlotorynski
Nature Reviews Molecular Cell Biology 15, 429

All News & Comment

Western blot articles from across Nature Portfolio

Latest Research and Reviews

Beovu, but not Lucentis impairs the function of the barrier formed by retinal endothelial cells in vitro

Epidermal Growth Factor Stimulates Transforming Growth Factor-Beta Receptor Type II Expression In Corneal Epithelial Cells

Dengue virus nonstructural 3 protein interacts directly with human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and reduces its glycolytic activity

Molecular details of secretory phospholipase A₂ from flax (Linum usitatissimum L.) provide insight into its structure and function

Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations

Single cell–resolution western blotting

News and Comment

Enhanced antibody validation

Should we ignore western blots when selecting antibodies for other applications?

Response to: Should we ignore western blots when selecting antibodies for other applications?

Single-cell western blotting

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