Two-hybrid system articles within Nature Communications

Featured

• Article
  03 April 2024 | Open Access

  ReLo is a simple and rapid colocalization assay to identify and characterize direct protein–protein interactions

  Characterising interactions between proteins that are large and poorly soluble remains challenging. Here, the authors describe ReLo, a rapid and versatile eukaryotic cell culture-based method for detecting and studying direct interactions between structurally complex proteins.

  • Harpreet Kaur Salgania
  • , Jutta Metz
  •  & Mandy Jeske

• Article
  25 August 2016 | Open Access

  A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4

  The centrosome is a large intracellular structure that serves as the microtubule-organising center, but how it is accurately assembled is not known. Here the authors generate a ‘domain-level’ centrosome interactome and show that Plk4 positions the essential centriole component Asterless by phosphorylating Cep135.

  • Brian J. Galletta
  • , Carey J. Fagerstrom
  •  & Nasser M. Rusan