Transgenic organisms articles within Nature Communications

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• Article
  13 September 2019 | Open Access

  Ubiquitination of RIPK1 suppresses programmed cell death by regulating RIPK1 kinase activation during embryogenesis

  RIPK1 integrates signals that drive both NF-κB activation and cell death pathways. Here Zhang et al. generate RIPK1 knock-in mice lacking a major ubiquitination site and demonstrate that this modification is important to suppress cell death during embryogenesis and inflammation postnatally.

  • Xixi Zhang
  • , Haiwei Zhang
  •  & Haibing Zhang

• Article
  21 September 2017 | Open Access

  Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis

  Clarifying the source of proteins in mixed biological environments, such as after transplantation or parabiosis, remains a challenge. Here, the authors address this need with a mouse strain that incorporates a methionine derivate into proteins, allowing for their detection using click chemistry and antibody arrays.

  • Yan Liu
  • , Michael J. Conboy
  •  & Irina M. Conboy

• Article
  05 September 2017 | Open Access

  Cre/lox-assisted non-invasive in vivo tracking of specific cell populations by positron emission tomography

  Non-invasive cell tracking is a powerful method to visualize cells in vivo under physiological and pathophysiological conditions. Here Thunemann et al. generate a mouse model for in vivo tracking and quantification of specific cell types by combining a PET reporter gene with Cre-dependent activation that can be exploited for any cell population for which a Cre mouse line is available.

  • Martin Thunemann
  • , Barbara F. Schörg
  •  & Robert Feil

• Article
  07 October 2015 | Open Access

  Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice

  Haem-free, NO-insensitive soluble guanylate cyclase (apo-sGC) generated during oxidative stress contributes to cardiovascular pathology. By generating and characterizing apo-sGC knock-in mice, Thoonen et al. provide a scientific ground for the therapeutic concept of sGC activators, and dissect the relevance of the NO-sGC axis.

  • Robrecht Thoonen
  • , Anje Cauwels
  •  & Peter Brouckaert

• Article
  19 November 2014 | Open Access

  Positron emission tomography and functional characterization of a complete PBR/TSPO knockout

  The 18-kDa translocator protein (TSPO) has been implicated in steroid biogenesis and neuroinflammation. Here, the authors create viable and fertile global TSPO knockout mice, challenging the assumption that TSPO is essential for mouse development but suggesting that it may have a role under certain disease conditions.

  • Richard B. Banati
  • , Ryan J. Middleton
  •  & Guo-Jun Liu

• Article
  21 August 2012 | Open Access

  Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases

  Hemimetabolous insects comprise many pests but introducing targeted mutations into these species has been difficult. This paper reports efficient targeted mutagenesis, and the generation of homozygous knockouts, in crickets based on zinc finger nucleases or transcription activator-like effector nucleases.

  • Takahito Watanabe
  • , Hiroshi Ochiai
  •  & Taro Mito