Transfection is a process by which foreign DNA is transferred to host cells. It includes any method for gene delivery but is usually used in the context of non-viral methods. Transfection can result in transient or stable genetic changes to the host (transformation).

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News and Comment

  • Comments & Opinion |

    Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels.

    • Toby J Gibson
    • , Markus Seiler
    •  & Reiner A Veitia
    Nature Methods 10, 715-721
  • Research Highlights |

    Precise amounts of DNA and quantum dots can be moved into cells through tiny channels.

    • Monya Baker
    Nature Methods 8, 996-997