Tissue culture articles from across Nature Portfolio

Pierre-Alexis Mouthuy and colleagues report the actuation of cell-material constructs in flexible bioreactor chambers inserted into musculoskeletal humanoid robots. This opens up the opportunity of applying realistic biomechanical stresses to tissue engineered constructs during culture.

The authors describe procedures for generating and biobanking glioblastoma organoids from patient tumor tissue and testing of chimeric antigen receptor T cell efficacy by co-culture. Tissue processing, immunohistology and detection of hypoxic gradients and actively proliferating cells are also described.

To recapitulate some of the complexities of the human physiology, engineered tissues can be linked by vascular flow in a multi-organ chip.

Researchers show that mouse stem and adult cells can develop into blastocyst-like structures.