Super-resolution microscopy

Super-resolution microscopy includes a variety of microscopy techniques that increase the resolving ability of a light microscope well beyond the classical limits dictated by the diffraction barrier.

Latest Research and Reviews

  • Research
    | Open Access

    Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale. Recently, the method called MINFLUX has achieved a ~10-fold improvement in spatial resolution over previously developed techniques, reaching molecular resolution with moderate photon counts. However, the technical complexity of this technique has hindered its widespread application. The new technique called RASTMIN provides equivalent resolution to MINFLUX while it can be implemented in standard scanning (confocal) microscopes.

    • Luciano A. Masullo
    • , Alan M. Szalai
    •  & Fernando D. Stefani
  • Research
    | Open Access

    Stress granules are non-membranous organelles connected to stress responses and age-related disease. Here, the authors identify a conserved yeast protein, Lsm7, that facilitates stress granule formation through dynamic liquid-liquid phase separation condensates upon 2-deoxy-D-glucose-induced stress.

    • Michelle Lindström
    • , Lihua Chen
    •  & Beidong Liu
  • Research
    | Open Access

    A subset of uropathogenic Escherichia coli are able to halt division and grow into highly filamentous cells during infection of bladder epithelial cells. Here, authors aim to determine the mechanism, and understand the dynamics of cell division machinery during infection-related filamentation.

    • Bill Söderström
    • , Matthew J. Pittorino
    •  & Iain G. Duggin
  • Research
    | Open Access

    Here, the authors generated an artificial RNA molecule, or aptamer, specific for the Amyotrophic Lateral Sclerosis protein TDP-43. By interacting avidly with its target, the aptamer can be exploited to track TDP-43 phase transition in vitro and in cells.

    • Elsa Zacco
    • , Owen Kantelberg
    •  & Gian Gaetano Tartaglia
  • Research
    | Open Access

    Pixel-to-pixel variations in sCMOS cameras lead to image artifacts in widefield fluorescence microscopy, and algorithmic corrections require thorough camera characterization. Here, the authors present an open source automated pipeline for camera characterization based solely on thermally generated signal.

    • Robin Diekmann
    • , Joran Deschamps
    •  & Jonas Ries

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