Super-resolution microscopy articles from across Nature Portfolio

Current expansion microscopy approaches need further optimization to achieve the precision of nanoscopy techniques. Here, the authors develop an iterative ultrastructure expansion microscopy (iU-ExM) approach that achieves SMLM-level resolution.

Monomeric and tandem dimer derivatives of the bright and photostable green fluorescent protein StayGold offer versatile tools for tagging target proteins and membranes in extended live-cell imaging.

Sanguina nivaloides thrives in red snowfields worldwide. It cannot be cultivated. Using environmental samples, 3D electron microscopy combined with functional analyzes revealed unique cell architecture features adapted to life in a snowy environment.

Leveraging a label-free interferometric scattering microscope, scientists tracked numerous cargos within a crowded cellular environment. Intriguingly, cells employ effective strategies echoing human transportation systems to manage such transportation hurdles.

We introduce GelMap, a flexible workflow for reporting deformations and anisotropy in expansion microscopy. By intrinsically calibrating the expansion hydrogel using a fluorescent grid that scales with expansion and deforms with anisotropy, GelMap enables the reliable quantification of expansion factors and correction of deformations.

An expansion microscopy technique called ChromExM offers detailed views into the organization chromatin and associated gene expression machinery in embryos.

The introduction of a two-step deconvolution workflow maximizes the detection of fluorescence in fluctuation-based super-resolution imaging, enabling a square millimetre field of view to be captured in as little as ten minutes.

We developed LIONESS, a technology that leverages improvements to optical super-resolution microscopy and prior information on sample structure via machine learning to overcome the limitations (in 3D-resolution, signal-to-noise ratio and light exposure) of optical microscopy of living biological specimens. LIONESS enables dense reconstruction of living brain tissue and morphodynamics visualization at the nanoscale.

Two papers in Science demonstrate tracking of the stepping motion of the kinesin motor protein with nanometric spatial precision and sub-millisecond temporal resolution by using MINFLUX, a highly photon-efficient single-molecule localization technique.