Stem cells

  • Article | | open

    Whether the Wnt enhanceosome’ components BCL9/9l can affect intestinal homeostasis and tumorigenesis is still unclear. Using conditional Bcl9/9l KO mice, the authors of this study show that the BCL9/9l complex is required for intestinal stem cells to drive tissue regeneration and that loss of BCL9/9l suppresses Wnt-driven transformation.

    • David M. Gay
    • , Rachel A. Ridgway
    • , Miryam Müeller
    • , Michael C. Hodder
    • , Ann Hedley
    • , William Clark
    • , Joshua D. Leach
    • , Rene Jackstadt
    • , Colin Nixon
    • , David J. Huels
    • , Andrew D. Campbell
    • , Thomas G. Bird
    •  & Owen J. Sansom
  • Article | | open

    BCL9 and Pygo are components of Wnt enhanceosome, which facilitates β-catenin-dependent transcription. Here, the authors show that deletion of Bcl9 and Pygo suppresses tumorigenesis and extends disease free survival in two different colorectal cancer models, suggesting a strategy for drugging β-catenin signalling in this cancer.

    • Juliusz Mieszczanek
    • , Laurens M. van Tienen
    • , Ashraf E. K. Ibrahim
    • , Douglas J. Winton
    •  & Mariann Bienz
  • Article | | open

    The diversity of fibroblasts contributing to wound healing is unclear. Here, the authors use single-cell RNA-sequencing to identify heterogeneity among murine fibroblasts in the wound and find that recruited myeloid cells contribute to adipocyte regeneration during healing.

    • Christian F. Guerrero-Juarez
    • , Priya H. Dedhia
    • , Suoqin Jin
    • , Rolando Ruiz-Vega
    • , Dennis Ma
    • , Yuchen Liu
    • , Kosuke Yamaga
    • , Olga Shestova
    • , Denise L. Gay
    • , Zaixin Yang
    • , Kai Kessenbrock
    • , Qing Nie
    • , Warren S. Pear
    • , George Cotsarelis
    •  & Maksim V. Plikus
  • Article | | open

    The pathways involved in exit from pluripotency in human embryonic stem cells are poorly understood. Here, the authors performed a CRISPR-based screen to identify genes that promote exit from naïve pluripotency and find a role for folliculin (FLCN) by regulating the mTOR and Wnt pathways.

    • J. Mathieu
    • , D. Detraux
    • , D. Kuppers
    • , Y. Wang
    • , C. Cavanaugh
    • , S. Sidhu
    • , S. Levy
    • , A. M. Robitaille
    • , A. Ferreccio
    • , T. Bottorff
    • , A. McAlister
    • , L. Somasundaram
    • , F. Artoni
    • , S. Battle
    • , R. D. Hawkins
    • , R. T. Moon
    • , C. B. Ware
    • , P. J. Paddison
    •  & H. Ruohola-Baker
  • Article | | open

    Hematopoietic stem cell (HSC) transplantation is a desirable treatment for many non-malignant and malignant diseases, but its use requires preconditioning of recipients with irradiation or chemotherapy that often induces high toxicity. Here the authors show that antibody-drug-conjugate to CD117, a HSC marker, allows specific and efficient preconditioning for HSC therapy.

    • Agnieszka Czechowicz
    • , Rahul Palchaudhuri
    • , Amelia Scheck
    • , Yu Hu
    • , Jonathan Hoggatt
    • , Borja Saez
    • , Wendy W. Pang
    • , Michael K. Mansour
    • , Tiffany A. Tate
    • , Yan Yi Chan
    • , Emily Walck
    • , Gerlinde Wernig
    • , Judith A. Shizuru
    • , Florian Winau
    • , David T. Scadden
    •  & Derrick J. Rossi
  • Article | | open

    Transplantation of allogeneic bone marrow helps establish chimerism that may induce tolerance to tissue grafts. Here the authors show that a CD117-antibody-drug-conjugate helps precondition the recipients for inducing mixed chimerism and allo-tolerance without clear adverse effects or the need for chronic immune suppression.

    • Zhanzhuo Li
    • , Agnieszka Czechowicz
    • , Amelia Scheck
    • , Derrick J. Rossi
    •  & Philip M. Murphy
  • Article | | open

    The use of pluripotent-stem cell derived organs for transplantation would be promising, if organs can be grown in a suitable host. Here, the authors use interspecific blastocyst complementation to generate a mouse pluripotent stem cell-derived kidney in anephric Sall1 mutant rats.

    • Teppei Goto
    • , Hiromasa Hara
    • , Makoto Sanbo
    • , Hideki Masaki
    • , Hideyuki Sato
    • , Tomoyuki Yamaguchi
    • , Shinichi Hochi
    • , Toshihiro Kobayashi
    • , Hiromitsu Nakauchi
    •  & Masumi Hirabayashi
  • Article | | open

    Mammalian joints have poor regenerative capacity following amputation. Here, the authors show that in mice, stimulation of the amputation wound with BMP2 and BMP9 stimulates regeneration of a synovial joint that includes bone, cartilage and a synovial cavity.

    • Ling Yu
    • , Lindsay A. Dawson
    • , Mingquan Yan
    • , Katherine Zimmel
    • , Yu-Lieh Lin
    • , Connor P. Dolan
    • , Manjong Han
    •  & Ken Muneoka
  • Article | | open

    High fat diets (HFD) are thought to perturb murine hematopoiesis as a result of obesity. Here the authors find that short-term HFD reduces hematopoietic stem cells (HSC), disrupts lipid rafts and TGF-β1 signalling. Injecting HFD-fed mice with recombinant TGF-β1 can rescue HSC loss.

    • François Hermetet
    • , Anne Buffière
    • , Aziza Aznague
    • , Jean-Paul Pais de Barros
    • , Jean-Noël Bastie
    • , Laurent Delva
    •  & Ronan Quéré
  • Article | | open

    Lineage segregation from conception to gastrulation has been mapped at the single cell level in mouse, human and monkey. Here, the authors provide a comprehensive analysis of porcine preimplantation development using single cell RNA-seq; mapping metabolic changes, X chromosome inactivation and signalling pathways.

    • Priscila Ramos-Ibeas
    • , Fei Sang
    • , Qifan Zhu
    • , Walfred W. C. Tang
    • , Sarah Withey
    • , Doris Klisch
    • , Liam Wood
    • , Matt Loose
    • , M. Azim Surani
    •  & Ramiro Alberio
  • Article | | open

    The precise cellular patterning and decisions of early embryogenesis have been hard to mimic in vitro. Here, the authors culture murine embryonic and trophoblast stem cells together with extra-embryonic endoderm stem cells to form embryo-like structures (ETX-embryoids), which can initiate an implantation response.

    • Shaopeng Zhang
    • , Tianzhi Chen
    • , Naixin Chen
    • , Dengfeng Gao
    • , Bingbo Shi
    • , Shuangbo Kong
    • , Rachel Claire West
    • , Ye Yuan
    • , Minglei Zhi
    • , Qingqing Wei
    • , Jinzhu Xiang
    • , Haiyuan Mu
    • , Liang Yue
    • , Xiaohua Lei
    • , Xuepeng Wang
    • , Liang Zhong
    • , Hui Liang
    • , Suying Cao
    • , Juan Carlos Izpisua Belmonte
    • , Haibin Wang
    •  & Jianyong Han
  • Article | | open

    Enhanced Wnt receptor activity is a major cause of cancer development. Here the authors identify camelid single-domain antibody fragments (VHHs) that bind to the Wnt receptor LRP5/6 ectodomain, determine the crystal structures and show that these VHHs selectively inhibit Wnt3- mediated cellular responses and block the growth of mutant Wnt-hypersensitive intestinal tumor organoids.

    • Nicola Fenderico
    • , Revina C. van Scherpenzeel
    • , Michael Goldflam
    • , Davide Proverbio
    • , Ingrid Jordens
    • , Tomica Kralj
    • , Sarah Stryeck
    • , Tarek Z. Bass
    • , Guy Hermans
    • , Christopher Ullman
    • , Teodor Aastrup
    • , Piet Gros
    •  & Madelon M. Maurice
  • Article | | open

    Myelodyplastic hematopoietic stem cells (MDS HSC) have eluded in vivo modeling. Here the authors present a highly efficient MDS patient-derived xenotransplantation model in cytokine-humanized mice with replication of the donors’ genetic complexity and myeloid, erythroid, and megakaryocytic lineage dysplasia.

    • Yuanbin Song
    • , Anthony Rongvaux
    • , Ashley Taylor
    • , Tingting Jiang
    • , Toma Tebaldi
    • , Kunthavai Balasubramanian
    • , Arun Bagale
    • , Yunus Kasim Terzi
    • , Rana Gbyli
    • , Xiaman Wang
    • , Xiaoying Fu
    • , Yimeng Gao
    • , Jun Zhao
    • , Nikolai Podoltsev
    • , Mina Xu
    • , Natalia Neparidze
    • , Ellice Wong
    • , Richard Torres
    • , Emanuela M. Bruscia
    • , Yuval Kluger
    • , Markus G. Manz
    • , Richard A. Flavell
    •  & Stephanie Halene
  • Article | | open

    Animal studies have shown that the nutritional status of parents can predispose the offspring to obesity and obesity-related diseases. Here the authors show that cardiac dysfunction induced by a high-fat diet persists for two generations in Drosophila, and that targeted expression of ATGL/bmm in the offspring, as well as inhibition of H3K27 trimethylation, is cardioprotective.

    • Maria Clara Guida
    • , Ryan Tyge Birse
    • , Alessandra Dall’Agnese
    • , Paula Coutinho Toto
    • , Soda Balla Diop
    • , Antonello Mai
    • , Peter D. Adams
    • , Pier Lorenzo Puri
    •  & Rolf Bodmer
  • Article | | open

    While human lifespan is only moderately heritable, “getting old” runs in families. Here, van den Berg et al. study mortality data from three-generation cohorts to define a threshold for longevity and find that individuals have an increasing survival advantage with each additional relative in the top 10% survivors of their birth cohort.

    • Niels van den Berg
    • , Mar Rodríguez-Girondo
    • , Ingrid K. van Dijk
    • , Rick J. Mourits
    • , Kees Mandemakers
    • , Angelique A. P. O. Janssens
    • , Marian Beekman
    • , Ken R. Smith
    •  & P. Eline Slagboom
  • Article | | open

    Transposable elements (TEs) fulfill essential but poorly understood roles in genome organization and gene expression control. Here the authors show that the regulation of TEs occurs through overlapping epigenetic mechanisms that control the expression and chromatin signatures at TEs.

    • Jiangping He
    • , Xiuling Fu
    • , Meng Zhang
    • , Fangfang He
    • , Wenjuan Li
    • , Mazid Md. Abdul
    • , Jianguo Zhou
    • , Li Sun
    • , Chen Chang
    • , Yuhao Li
    • , He Liu
    • , Kaixin Wu
    • , Isaac A. Babarinde
    • , Qiang Zhuang
    • , Yuin-Han Loh
    • , Jiekai Chen
    • , Miguel A. Esteban
    •  & Andrew P. Hutchins
  • Article | | open

    The inactive X chromosome (Xi) has an atypical structure, with global loss of TADs, A/B compartments and formation of mega-domains. Here the authors show that the non-canonical SMC family protein, SmcHD1, important for developmental gene silencing on Xi, antagonises TAD formation and compartmentalization on the Xi in a transcription independent way.

    • Michal R. Gdula
    • , Tatyana B. Nesterova
    • , Greta Pintacuda
    • , Jonathan Godwin
    • , Ye Zhan
    • , Hakan Ozadam
    • , Michael McClellan
    • , Daniella Moralli
    • , Felix Krueger
    • , Catherine M. Green
    • , Wolf Reik
    • , Skirmantas Kriaucionis
    • , Edith Heard
    • , Job Dekker
    •  & Neil Brockdorff
  • Article | | open

    The source of where definitive hematopoietic stem cells (dHSCs) develop in the mouse embyro has been much debated. Here, the authors identify that dHSCs arise from the intra-embyronic region by using murine embryonic explant transplantation but not from the yolk sac.

    • Miguel Ganuza
    • , Ashley Chabot
    • , Xing Tang
    • , Wenjian Bi
    • , Sivaraman Natarajan
    • , Robert Carter
    • , Charles Gawad
    • , Guolian Kang
    • , Yong Cheng
    •  & Shannon McKinney-Freeman
  • Article | | open

    Human embryonic stem cells can be differentiated in vitro into primordial germ cell-like cells (PGCLCs) that resemble early primordial germ cells (PGCs). Here the authors transplant PGCLCs generated from rhesus macaque iPSCs into mouse and rhesus macaque seminiferous tubules, which matures these into late PGCs and spermatogonia-like cells.

    • Enrique Sosa
    • , Di Chen
    • , Ernesto J. Rojas
    • , Jon D. Hennebold
    • , Karen A. Peters
    • , Zhuang Wu
    • , Truong N. Lam
    • , Jennifer M. Mitchell
    • , Meena Sukhwani
    • , Ramesh C. Tailor
    • , Marvin L. Meistrich
    • , Kyle E. Orwig
    • , Gunapala Shetty
    •  & Amander T. Clark
  • Article | | open

    In Arabidopsis, the AG and CRC transcription factors terminate floral stem cells and allow the emergence of female floral organs. Here the authors show that AG and CRC form a feed-forward loop that controls local auxin biosynthesis via induction of YUCCA4 to ensure successful gynoecium formation.

    • Nobutoshi Yamaguchi
    • , Jiangbo Huang
    • , Yoshitaka Tatsumi
    • , Masato Abe
    • , Shigeo S. Sugano
    • , Mikiko Kojima
    • , Yumiko Takebayashi
    • , Takatoshi Kiba
    • , Ryusuke Yokoyama
    • , Kazuhiko Nishitani
    • , Hitoshi Sakakibara
    •  & Toshiro Ito
  • Article | | open

    Acute myeloid leukaemia (AML) affects people of all ages. Here, the authors model AML in vivo and demonstrate that the age of the cell of origin impacts leukaemia development and the genetic signature where adult cells of origin give rise exclusively to AML and young cells of origin give rise to myeloid, lymphoid or mixed phenotype acute leukaemia.

    • Shahzya Chaudhury
    • , Caitríona O’Connor
    • , Ana Cañete
    • , Joana Bittencourt-Silvestre
    • , Evgenia Sarrou
    • , Áine Prendergast
    • , Jarny Choi
    • , Pamela Johnston
    • , Christine A. Wells
    • , Brenda Gibson
    •  & Karen Keeshan
  • Article | | open

    HMGN1 and HMGN2 are ubiquitous nucleosome binding proteins. Here the authors provide evidence that HMGN proteins preferentially localize to chromatin regulatory sites to modulate the plasticity of the epigenetic landscape, proposing that HGMNs stabilize, rather than determine, cell identity.

    • Bing He
    • , Tao Deng
    • , Iris Zhu
    • , Takashi Furusawa
    • , Shaofei Zhang
    • , Wei Tang
    • , Yuri Postnikov
    • , Stefan Ambs
    • , Caiyi Cherry Li
    • , Ferenc Livak
    • , David Landsman
    •  & Michael Bustin
  • Article | | open

    The rate of repair declines with age; however, exposure to young circulations can rejuvenate fracture repair, but how this is accomplished is unknown. Here, the authors identify proteins, including low-density lipoprotein receptor-related protein 1 (Lrp1), as being secreted from young macrophages and rejuvenating fracture repair in mice.

    • Linda Vi
    • , Gurpreet S. Baht
    • , Erik J. Soderblom
    • , Heather Whetstone
    • , Qingxia Wei
    • , Bridgette Furman
    • , Vijitha Puviindran
    • , Puviindran Nadesan
    • , Matthew Foster
    • , Raymond Poon
    • , James P. White
    • , Yasuhito Yahara
    • , Adeline Ng
    • , Tomasa Barrientos
    • , Marc Grynpas
    • , M. Arthur Mosely
    •  & Benjamin A. Alman
  • Article | | open

    Studies examining human podocytopathies have utilised 2D cultured primary or immortalised podocyte cell lines. Here, the authors demonstrate that 3D human glomeruli sieved from induced pluripotent stem cell-derived kidney organoids retain an improved podocyte identity in vitro facilitating disease modelling and toxicity testing.

    • Lorna J. Hale
    • , Sara E. Howden
    • , Belinda Phipson
    • , Andrew Lonsdale
    • , Pei X. Er
    • , Irene Ghobrial
    • , Salman Hosawi
    • , Sean Wilson
    • , Kynan T. Lawlor
    • , Shahnaz Khan
    • , Alicia Oshlack
    • , Catherine Quinlan
    • , Rachel Lennon
    •  & Melissa H. Little
  • Article | | open

    Limb regeneration requires a blastema with progenitor cells, immune cells, and an overlying wound epidermis, but molecular identities of these populations are unclear. Here, the authors use single-cell RNA-sequencing to identify transcriptionally distinct cell populations in adult axolotl limb blastemas.

    • Nicholas D. Leigh
    • , Garrett S. Dunlap
    • , Kimberly Johnson
    • , Rachelle Mariano
    • , Rachel Oshiro
    • , Alan Y. Wong
    • , Donald M. Bryant
    • , Bess M. Miller
    • , Alex Ratner
    • , Andy Chen
    • , William W. Ye
    • , Brian J. Haas
    •  & Jessica L. Whited
  • Article | | open

    Royal jelly is the queen-maker for the honey bee that also has effects on longevity, fertility, and regeneration in mammals. Here the authors provide evidence that its major protein component Royalactin, and the mammalian structural analog Regina, maintain pluripotency in mouse ESCs by activating a ground-state pluripotency-like gene network.

    • Derrick C. Wan
    • , Stefanie L. Morgan
    • , Andrew L. Spencley
    • , Natasha Mariano
    • , Erin Y. Chang
    • , Gautam Shankar
    • , Yunhai Luo
    • , Ted H. Li
    • , Dana Huh
    • , Star K. Huynh
    • , Jasmine M. Garcia
    • , Cole M. Dovey
    • , Jennifer Lumb
    • , Ling Liu
    • , Katharine V. Brown
    • , Abel Bermudez
    • , Richard Luong
    • , Hong Zeng
    • , Victoria L. Mascetti
    • , Sharon J. Pitteri
    • , Jordon Wang
    • , Hua Tu
    • , Marco Quarta
    • , Vittorio Sebastiano
    • , Roel Nusse
    • , Thomas A. Rando
    • , Jan E. Carette
    • , J. Fernando Bazan
    •  & Kevin C. Wang
  • Article | | open

    Reprogramming of mitochondria metabolism occurs during naïve to primed pluripotency differentiation in mouse embryonic stem cells (ESCs). Here the authors show that mitochondrial MTCH2 regulates mitochondrial fusion and that this fusion is required for naïve to primed pluripotency conversion

    • Amir Bahat
    • , Andres Goldman
    • , Yehudit Zaltsman
    • , Dilshad H. Khan
    • , Coral Halperin
    • , Emmanuel Amzallag
    • , Vladislav Krupalnik
    • , Michael Mullokandov
    • , Alon Silberman
    • , Ayelet Erez
    • , Aaron D. Schimmer
    • , Jacob H. Hanna
    •  & Atan Gross
  • Article | | open

    “Satellite cells are crucial for skeletal muscle regeneration. Here the authors show that immunoglobulin superfamily containing leucine-rich repeat (Islr) promotes skeletal muscle regeneration via a mechanism involving Dishevelled-2 stabilization in satellite cells and protection from autophagy.

    • Kuo Zhang
    • , Yuying Zhang
    • , Lijie Gu
    • , Miaomiao Lan
    • , Chuncheng Liu
    • , Meng Wang
    • , Yang Su
    • , Mengxu Ge
    • , Tongtong Wang
    • , Yingying Yu
    • , Chang Liu
    • , Lei Li
    • , Qiuyan Li
    • , Yaofeng Zhao
    • , Zhengquan Yu
    • , Fudi Wang
    • , Ning Li
    •  & Qingyong Meng
  • Article | | open

    A role for Slit/Robo signalling in pancreas development has not been defined. Here, the authors generate Robo1/Robo2 knockout mice to identify both as needed for establishing initial pancreatic cell identity as well as for proliferation and survival of pancreatic progenitors.

    • Sophie Escot
    • , David Willnow
    • , Heike Naumann
    • , Silvia Di Francescantonio
    •  & Francesca M. Spagnoli
  • Article | | open

    Organ regeneration by stem cells is required to compensate for tissue damage during aging, although how stem cells are maintained in adulthood is poorly understood. Here, the authors show in Drosophila that Shavenbaby interacts with Yorkie, a mediator of Hippo signalling, to ensure adult stem cell survival.

    • Jérôme Bohère
    • , Alexandra Mancheno-Ferris
    • , Sandy Al Hayek
    • , Jennifer Zanet
    • , Philippe Valenti
    • , Kohsuke Akino
    • , Yuya Yamabe
    • , Sachi Inagaki
    • , Hélène Chanut-Delalande
    • , Serge Plaza
    • , Yuji Kageyama
    • , Dani Osman
    • , Cédric Polesello
    •  & François Payre
  • Article | | open

    Radiotherapy is the main treatment for most cancer, but it is unclear if targeting tumour vasculature can enhance tumour radiosensitivity. Here, the authors show that tumour endothelial-mesenchymal transition after radiotherapy leads to proliferation of radioresistant CSCs and tumour associated macrophages polarization.

    • Seo-Hyun Choi
    • , A-Ram Kim
    • , Jae-Kyung Nam
    • , Jin-Mo Kim
    • , Jee-Youn Kim
    • , Haeng Ran Seo
    • , Hae-June Lee
    • , Jaeho Cho
    •  & Yoon-Jin Lee
  • Article | | open

    In vitro culture has detrimental effects on transcriptomes and epigenetic programming of zygotes. Here the authors use microfluidic technology to co-culture bovine oviduct epithelial cells with zygotes and show that the transcriptomes and global methylation patterns of these zygotes are more similar to in vivo zygotes than to conventionally cultured zygotes.

    • Marcia A. M. M. Ferraz
    • , Hoon Suk Rho
    • , Daiane Hemerich
    • , Heiko H. W. Henning
    • , Helena T. A. van Tol
    • , Michael Hölker
    • , Urban Besenfelder
    • , Michal Mokry
    • , Peter L. A. M. Vos
    • , Tom A. E. Stout
    • , Séverine Le Gac
    •  & Bart M. Gadella
  • Article | | open

    Human induced pluripotent stem cell derived cardiomyocytes are a powerful model for cardiogenesis and disease in vitro. Here the authors comprehensively map cardiac differentiation using multiple modalities, including single-cell RNA seq and CyTOF, in cells with a gain  or loss of function in key cardiac transcription factors.

    • Jared M. Churko
    • , Priyanka Garg
    • , Barbara Treutlein
    • , Meenakshi Venkatasubramanian
    • , Haodi Wu
    • , Jaecheol Lee
    • , Quinton N. Wessells
    • , Shih-Yu Chen
    • , Wen-Yi Chen
    • , Kashish Chetal
    • , Gary Mantalas
    • , Norma Neff
    • , Eric Jabart
    • , Arun Sharma
    • , Garry P. Nolan
    • , Nathan Salomonis
    •  & Joseph C. Wu
  • Article | | open

    Cardiac progenitor cells (CPCs) form cardiomyocytes, pericytes, smooth muscle and endothelial cells during embryonic development. Here, the authors characterize mouse CPCs marked by Nkx2.5 and Isl1 from E7.5 to E9.5 by single cell RNA-seq and ATAC-seq, showing fate transitions involve distinct open chromatin state.

    • Guangshuai Jia
    • , Jens Preussner
    • , Xi Chen
    • , Stefan Guenther
    • , Xuejun Yuan
    • , Michail Yekelchyk
    • , Carsten Kuenne
    • , Mario Looso
    • , Yonggang Zhou
    • , Sarah Teichmann
    •  & Thomas Braun
  • Article | | open

    While young muscle faithfully regenerates damaged myofibers, aged muscle is impaired. Here the authors show the “anti-aging” protein α-Klotho is upregulated in young muscle after damage via promoter demethylation and this regulation is lost in aging, resulting in mitochondrial damage and an impaired healing response.

    • A. Sahu
    • , H. Mamiya
    • , S. N. Shinde
    • , A. Cheikhi
    • , L. L. Winter
    • , N. V. Vo
    • , D. Stolz
    • , V. Roginskaya
    • , W. Y. Tang
    • , C. St. Croix
    • , L. H. Sanders
    • , M. Franti
    • , B. Van Houten
    • , T. A. Rando
    • , A. Barchowsky
    •  & F. Ambrosio
  • Article | | open

    The effects of epigenetic regulators on different tumor cell populations can affect their potential as anticancer targets. In this study, the authors demonstrate that the histone methyltransferase G9a is a suppressor of lung tumor-propagating cells and tumor progression, acting through chromatin modification with MMP10 as one of its targets for metastasis regulation.

    • S. P. Rowbotham
    • , F. Li
    • , A. F. M. Dost
    • , S. M. Louie
    • , B. P. Marsh
    • , P. Pessina
    • , C. R. Anbarasu
    • , C. F. Brainson
    • , S. J. Tuminello
    • , A. Lieberman
    • , S. Ryeom
    • , T. M. Schlaeger
    • , B. J. Aronow
    • , H. Watanabe
    • , K. K. Wong
    •  & C. F. Kim
  • Article | | open

    Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that loss of the gene GSTT1 confers hypersensitivity.

    • Ting Zhou
    • , Tae Wan Kim
    • , Chi Nok Chong
    • , Lei Tan
    • , Sadaf Amin
    • , Zohreh Sadat Badieyan
    • , Suranjit Mukherjee
    • , Zaniar Ghazizadeh
    • , Hui Zeng
    • , Min Guo
    • , Miguel Crespo
    • , Tuo Zhang
    • , Reyn Kenyon
    • , Christopher L. Robinson
    • , Effie Apostolou
    • , Hui Wang
    • , Jenny Zhaoying Xiang
    • , Todd Evans
    • , Lorenz Studer
    •  & Shuibing Chen
  • Article | | open

    mRNA modifications have been shown to regulate mammalian development and disease. Here the authors show that the m6A methyltransferase Mettl3 ensures translational efficiency of the mesenchymal stem cell lineage allocator Pth1r, promoting osteogenesis and protecting from osteoporosis.

    • Yunshu Wu
    • , Liang Xie
    • , Mengyuan Wang
    • , Qiuchan Xiong
    • , Yuchen Guo
    • , Yu Liang
    • , Jing Li
    • , Rui Sheng
    • , Peng Deng
    • , Yuan Wang
    • , Rixin Zheng
    • , Yizhou Jiang
    • , Ling Ye
    • , Qianming Chen
    • , Xuedong Zhou
    • , Shuibin Lin
    •  & Quan Yuan
  • Article | | open

    The mechanisms regulating intestinal stem cell elimination remain unclear. Here, the authors identify that the pro-apoptotic protein ARTS (a Septin4 isoform) interacts with XIAP in the intestinal stem cell niche to  regulate stem cell survival during intestinal homeostasis and regeneration.

    • Elle Koren
    • , Yahav Yosefzon
    • , Roi Ankawa
    • , Despina Soteriou
    • , Avi Jacob
    • , Alexander Nevelsky
    • , Rahamim Ben-Yosef
    • , Gil Bar-Sela
    •  & Yaron Fuchs
  • Article | | open

    The NuRD complex plays an important role in regulating lineage commitment and cell fate during early embryonic development. Here the authors present an integrative analysis of MBD3/NuRD composition and binding in mouse embryonic stem cells and neural progenitor cells, providing a molecular basis for genome-wide NuRD localization

    • Susan L. Kloet
    • , Ino D. Karemaker
    • , Lisa van Voorthuijsen
    • , Rik G. H. Lindeboom
    • , Marijke P. Baltissen
    • , Raghu R. Edupuganti
    • , Deepani W. Poramba-Liyanage
    • , Pascal W. T. C. Jansen
    •  & Michiel Vermeulen
  • Article | | open

    In vitro expansion of human epidermal keratinocytes to resurface severe wound defects still relies on a human/mouse xenograft culture system. Here the authors develop a fully human, xeno-free culture system using skin-associated laminins, normally present in vivo, to replace mouse feeder cells.

    • Monica Suryana Tjin
    • , Alvin Wen Choong Chua
    • , Aida Moreno-Moral
    • , Li Yen Chong
    • , Po Yin Tang
    • , Nathan Peter Harmston
    • , Zuhua Cai
    • , Enrico Petretto
    • , Bien Keem Tan
    •  & Karl Tryggvason
  • Article | | open

    Leydig cells are steroidogenic cells in the testes and produce the androgens required for male development and spermatogenesis. Here the authors show that a multipotent progenitor population producing Leydig cells, pericytes and smooth muscle cells is maintained in a perivascular niche within the mouse fetal testis.

    • Deepti L. Kumar
    •  & Tony DeFalco
  • Article | | open

    Myoblast fusion is an essential step in muscle growth and regeneration, and is regulated by the G-protein coupled receptor (GPCR) BAI3. Here Hamoud et al. show that the GPCR activity of BAI3 is spatiotemporally regulated during myoblast fusion, and identify C1qL4 and Stabilin-2 as, respectively, negative and positive regulators of its activity.

    • Noumeira Hamoud
    • , Viviane Tran
    • , Takahiro Aimi
    • , Wataru Kakegawa
    • , Sylvie Lahaie
    • , Marie-Pier Thibault
    • , Ariane Pelletier
    • , G. William Wong
    • , In-San Kim
    • , Artur Kania
    • , Michisuke Yuzaki
    • , Michel Bouvier
    •  & Jean-François Côté
  • Article | | open

    A bioengineering approach to enhance the regeneration of large bone defects is lacking. Here, the authors show that a biomaterial scaffold with a channel-like pore architecture enables organized endochondral ossification through directional cell recruitment and extracellular matrix alignment.

    • A. Petersen
    • , A. Princ
    • , G. Korus
    • , A. Ellinghaus
    • , H. Leemhuis
    • , A. Herrera
    • , A. Klaumünzer
    • , S. Schreivogel
    • , A. Woloszyk
    • , K. Schmidt-Bleek
    • , S. Geissler
    • , I. Heschel
    •  & G. N. Duda
  • Article | | open

    Unbiased large scale screening of small molecules for drug discovery in psychiatric disease is technically challenging and financially costly. Here, Readhead and colleagues integrate in silico and in vitro approaches to design and conduct transcriptomic drug screening in schizophrenia patient-derived neural cells, in order to survey novel pathologies and points of intervention.

    • Benjamin Readhead
    • , Brigham J. Hartley
    • , Brian J. Eastwood
    • , David A. Collier
    • , David Evans
    • , Richard Farias
    • , Ching He
    • , Gabriel Hoffman
    • , Pamela Sklar
    • , Joel T. Dudley
    • , Eric E. Schadt
    • , Radoslav Savić
    •  & Kristen J. Brennand
  • Article | | open

    The transcription factor DAF-16/FOXO is a downstream effector of insulin/insulin-like growth factor signaling and plays an important role in stress resistance and longevity. Here, the authors show that DAF-16/FOXO can form a complex with HLH-30/TFEB to synergistically regulate transcription of target genes in response to certain stress stimuli.

    • Xin-Xuan Lin
    • , Ilke Sen
    • , Georges E. Janssens
    • , Xin Zhou
    • , Bryan R. Fonslow
    • , Daniel Edgar
    • , Nicholas Stroustrup
    • , Peter Swoboda
    • , John R. Yates 3rd
    • , Gary Ruvkun
    •  & Christian G. Riedel
  • Article | | open

    Self-renewal and pluripotency of human embryonic stem cells (hESCs) depends upon the function of the transcription factor OCT4. Here, the authors identified proteins associated with the OCT4 enhancer, notably ZNF207 that maintains both pluripotency and differentiation towards ectoderm through isoform switching.

    • Fang Fang
    • , Ninuo Xia
    • , Benjamin Angulo
    • , Joseph Carey
    • , Zackery Cady
    • , Jens Durruthy-Durruthy
    • , Theo Bennett
    • , Vittorio Sebastiano
    •  & Renee A. Reijo Pera
  • Article | | open

    Self-renewal of spermatogonial stem cells (SSC) is necessary for spermatogenesis and male fertility. Here the authors identify testicular endothelial cells (TECs) as a source of 5 key growth factors for self-renewal and expansion of human and mouse SSCs.

    • Dong Ha Bhang
    • , Bang-Jin Kim
    • , Byung Gak Kim
    • , Keri Schadler
    • , Kwan-Hyuck Baek
    • , Yong Hee Kim
    • , Wayland Hsiao
    • , Bi-Sen Ding
    • , Shahin Rafii
    • , Mitchell J. Weiss
    • , Stella T. Chou
    • , Thomas F. Kolon
    • , Jill P. Ginsberg
    • , Buom-Yong Ryu
    •  & Sandra Ryeom
  • Article | | open

    The early molecular events that ultimately lead to neuronal cell death in pathologies such as Parkinson’s disease are poorly understood. Here the authors use pluripotent stem-cell-derived human midbrain neurons and chemical biology tools to gain molecular level insight into the events induced by toxic and genetic stresses that mimic those occurring during neurodegeneration.

    • Sarah Kishinevsky
    • , Tai Wang
    • , Anna Rodina
    • , Sun Young Chung
    • , Chao Xu
    • , John Philip
    • , Tony Taldone
    • , Suhasini Joshi
    • , Mary L. Alpaugh
    • , Alexander Bolaender
    • , Simon Gutbier
    • , Davinder Sandhu
    • , Faranak Fattahi
    • , Bastian Zimmer
    • , Smit K. Shah
    • , Elizabeth Chang
    • , Carmen Inda
    • , John Koren 3rd
    • , Nathalie G. Saurat
    • , Marcel Leist
    • , Steven S. Gross
    • , Venkatraman E. Seshan
    • , Christine Klein
    • , Mark J. Tomishima
    • , Hediye Erdjument-Bromage
    • , Thomas A. Neubert
    • , Ronald C. Henrickson
    • , Gabriela Chiosis
    •  & Lorenz Studer