Stem-cell differentiation articles from across Nature Portfolio

Characterization of nuclear and nucleolar dynamics during adipogenic differentiation of human adipose stem cells points to nucleolar remodeling as an active, mechanoregulated mechanism triggered by cytoskeleton rearrangement.

Specification of neuroectoderm from TET3 enzymatic deficient and knockout embryonic stem cells reveals distinct catalytic-dependent and -independent roles of TET3 in activation of neural and suppression of mesodermal programs, respectively.

We present a protocol for achieving efficient generation of hPSC-CM aggregates in suspension culture, emphasizing process simplicity, robustness and GMP compliance. The strategy promotes clinical translation and other applications that require large numbers of CMs.

The underrepresentation of functional glial cells is a major challenge in brain organoid models. We developed an astroglia-enriched cortical organoid model that allows efficient generation of functional astrocytes and enables the formation of astroglial morphological subclasses with layer-specific gene expression profiles upon transplantation into the mouse brain.

Motivated by a CRISPR screen, in vitro and in vivo studies identified an essential role for the bromodomain and extraterminal domain (BET) family member BRD4 in the differentiation of second heart field progenitors into cardiomyocytes. Single-cell transcriptomic studies showed that BRD4 deficiency affects a specific subset of cardiac progenitor cells.

In vivo developmental atlases provide a crucial reference for the new class of stem-cell-derived human embryo models, helping accelerate insights into the mechanisms of human development.

A new method models mammary gland in vitro during physiological and pathological conditions.

In this Tools of the Trade article, Emmanuel Olivier describes an approach to reprogramming AML cancer cells into induced pluripotent stem cells, which provide faithful genetic models of the most common genetic subgroups of AML.