Featured
-
-
Article
| Open Access
Absolute protein quantification using fluorescence measurements with FPCountR
A challenge in synthetic biology is the empirical characterisation of genetic parts. Here the authors present FPCountR, a validated method and accompanying R package that enables the precise quantification of fluorescent protein reporters per bacterial cell to be enumerated in ‘proteins per cell’ or nanomolar units without requiring protein purification.
- Eszter Csibra
- & Guy-Bart Stan
-
Article
| Open Access
Global diversity dynamics in the fossil record are regionally heterogeneous
Global diversity trends in the fossil record vary regionally through time and space, affecting our ability to interpret macroevolutionary history. Here, the authors propose a method to eliminate spatial sampling bias, estimate origination and extinction rates, and generate diversity estimates, applying this method to the Late Permian to Early Jurassic marine fossil record.
- Joseph T. Flannery-Sutherland
- , Daniele Silvestro
- & Michael J. Benton
-
Perspective
| Open Access
A proteomics sample metadata representation for multiomics integration and big data analysis
The number of publicly available proteomics datasets is growing rapidly, but a standardized approach for describing the associated metadata is lacking. Here, the authors propose a format and a software pipeline to present and validate metadata, and integrate them into ProteomeXchange repositories.
- Chengxin Dai
- , Anja Füllgrabe
- & Yasset Perez-Riverol
-
Article
| Open Access
MuSyC is a consensus framework that unifies multi-drug synergy metrics for combinatorial drug discovery
The lack of a unifying metric characterizing combinatorial drug interactions has impeded the development of combinatorial therapies. Here, the authors present MuSyC, a consensus synergy metric that overcomes several caveats associated with other, popular metrics.
- David J. Wooten
- , Christian T. Meyer
- & Carlos F. Lopez
-
Perspective
| Open Access
Towards a unified open access dataset of molecular interactions
The IMEx consortium provides one of the largest resources of curated, experimentally verified molecular interaction data. Here, the authors review how IMEx evolved into a fundamental resource for life scientists and describe how IMEx data can support biomedical research.
- Pablo Porras
- , Elisabet Barrera
- & Sandra Orchard
-
Article
| Open Access
Cell-free prediction of protein expression costs for growing cells
The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.
- Olivier Borkowski
- , Carlos Bricio
- & Tom Ellis
Four-dimensional trapped ion mobility spectrometry lipidomics for high throughput clinical profiling of human blood samples