Single-molecule fluorescence

Single-molecule fluorescence experiments involve the study of individual fluorescent molecules, and can provide information that would be hidden in measurements involving a large number of molecules. Techniques for probing single-molecule fluorescence include total internal reflection fluorescence microscopy (TIRF microscopy).

Latest Research and Reviews

  • Research
    | Open Access

    Increasing the rigidity of a macromolecule while maintaining solubility is challenging. Here, the authors demonstrate covalent connection of two rigid-rod polymer chains with stiff connectors, leading to rigid ladder structures with well-defined conjugated rails.

    • Stefanie A. Meißner
    • , Theresa Eder
    •  & Sigurd Höger
  • Research
    | Open Access

    Molecular designs improving the performance of thermally activated delayed fluorescence (TADF) emitters are greatly desired for optoelectronic applications. The authors propose a molecular geometry with donor and acceptor moieties facing each other separated by an anthracene bridge, giving rise to hot exciplex states producing color pure and fast TADF emission.

    • A. Lennart Schleper
    • , Kenichi Goushi
    •  & Alexander J. C. Kuehne
  • Research
    | Open Access

    Oligofurans have attracted great attention because of their strong fluorescence, charge delocalization, and increased solubility. Here the authors show a bottom-up modular construction of chemically and structurally well-defined oligo(arylfuran)s by de novo synthesis of α,β′-bifuran monomers and late-stage bromination, stannylation and subsequent coupling reaction.

    • Yang Chen
    • , Pingchuan Shen
    •  & Shifa Zhu
  • Research
    | Open Access

    Commonly, large π-conjugated systems facilitate low-energy electronic transitions. Here, the authors demonstrate that the relief of excited-state antiaromaticity of the benzene core leads to large Stokes shifts, and allows the construction of emitters covering the entire visible spectrum without the need of extending π-conjugation.

    • Heechan Kim
    • , Woojin Park
    •  & Dongwhan Lee
  • Research |

    Energy funnelling within multichromophoric assemblies is key to the conversion of solar energy by plants. Now, energy transport between phthalocyanine-based chromophores has been monitored at the submolecular level using scanning tunnelling microscopy, focusing on the role of ancillary, passive and blocking chromophores in promoting and directing energy transfer between distant donor and acceptor units.

    • Shuiyan Cao
    • , Anna Rosławska
    •  & Guillaume Schull
    Nature Chemistry 13, 766-770

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