Single-molecule fluorescence articles from across Nature Portfolio

A multi-view reflector microscope based on polarization modulation and pupil splitting enables single-molecule orientation-localization microscopy with precisions of 10.9 nm and 2.0°.

The development of photochromic systems is an important and growing area of research, in particular for bioactive molecular photoswitches. Here, the authors report on photopharmacological antimitotic agents, operational under visible light, based on a peptide-derived hemipiperazine photochrome.

The develop of organic functional materials requires the exploration of the pressure dependent emissive mechanisms of molecular structures, conformations and stacking modes. Here, Tong et al propose a strategy for monitoring the pressure-induced fluorescence under multiple excitation channels.

The design of photoactivatable fluorophores—which are required for some super-resolution fluorescence microscopy methods—usually relies on light-sensitive protecting groups imparting lipophilicity and generating reactive by-products. Now, it has been shown that by exploiting a unique intramolecular photocyclization, bright and highly photostable fluorophores can be rapidly generated in situ from appropriately substituted 1-alkenyl-3,6-diaminoxanthone precursors.

The development of advanced materials for information encryption is desirable. Here, the authors report materials that enable orthogonal and temporal encryption of information via dynamic assembly-induced multicolor pyrene-based supramolecular systems.