Single-cell imaging articles from across Nature Portfolio

A live-cell trajectory embedding analysis characterizes the morphodynamical changes associated with molecular and ligand-induced responses and improves identification of metastable cell states compared to morphological snapshot-based analysis.

To understand why genetically identical cells die at different times the authors measured damage dynamics in individual cells. They report lifespan variation comes not from initial conditions but from stochastic accumulation of damage that saturates repair systems.

Takhaveev et al. use single-cell methods and mathematical modeling to reveal distinct waves in the synthesis rates of proteins, lipids and polysaccharides during the budding yeast cell cycle and relate them to flux changes in primary metabolism.

A high-content screening assay based on a comprehensive set of cytological features, together with a robust statistical analysis workflow for profiling broad-based cellular phenotypic responses to small molecules or genetic perturbations.

Monitoring the aging process in vivo is challenging. Here the authors generate a Glb1^+/m‒Glb1-2A-mCherry (GAC) reporter mouse model, where the GAC signal is consistently correlated with established biomarkers of cellular senescence, cardiac hypertrophy and shortened lifespan, which may prove helpful for studies developing anti-aging interventions.