RNAi articles from across Nature Portfolio

DNA recognition by Argonaute proteins activates effector nucleases that trigger indiscriminate DNA and RNA cleavage and induce abortive infection.

miR408-5p typically regulates target IAA30 via translation repression, but switches to cleaving IAA30 mRNA under high auxin conditions. miR393, miR156, miR408-5p and their targets could hierarchically act in auxin pathway and regulate leaf inclination.

With six approved drugs, siRNA is now an established therapeutic modality poised for expansion.

The lack of control over Cas13 activity has limited its utility. Here the authors report Control of RNA with Inducible SpliT CAs13 Orthologs and Exogenous Ligands (CRISTAL), controlled by orthogonal split inducible Cas13 effectors that can be turned ON or OFF, providing precise temporal control.

Joana Vidigal reminds us of the first paper to report an endogenous role of the nucleolytic activity of the mammalian RNAi protein argonaute-2.

Cell quiescence dampens mTOR signalling and enables AGO2 localization to the nucleus, where it suppresses retrotransposons by directly cleaving their RNA.

Across multiple cancer types, hotspot mutations in SF3B1 confer selective sensitivity to multiple clinically available PARP inhibitors. This sensitivity is due to reduced levels of CINP specifically in SF3B1-mutant cells, which leads to a loss of the canonical replication stress response after challenge with PARP inhibitors.