RNA splicing articles from across Nature Portfolio

RNA splicing is a modification of the nascent pre-messenger RNA (pre-mRNA) transcript in which introns are removed and exons are joined prior to translation. For many eukaryotic introns, splicing is done in a series of reactions which are catalyzed by the spliceosome, a complex of small nuclear ribonucleoproteins (snRNPs) and associated proteins, but there are also self-splicing introns.

Latest Research and Reviews

News and Comment

  • Research Highlights |

    Two studies have revealed that the characteristic distribution of N6-methyladenosine (m6A) — an RNA modification known to be functionally important for mRNA metabolism among other processes — in mRNA is shaped by the exon junction complex during splicing.

    • Dorothy Clyde
  • Comments & Opinion |

    Long-read sequencing has become a widely employed technology that enables a comprehensive view of RNA transcripts. Here, we discuss the importance of long-read sequencing in interpreting the variables along RNA molecules, such as polyadenylation sites, transcription start sites, splice sites and other RNA modifications. In addition, we highlight the history of short-read and long-read technologies and their advantages and disadvantages, as well as future directions in the field.

    • Careen Foord
    • , Justine Hsu
    •  & Hagen U. Tilgner
    Nature Methods 20, 20-24
  • News & Views |

    Several new technologies have used synthetic RNAs that leverage the cell’s RNA splicing machinery to drive the expression of gene products. A new study now reports a technique to dynamically and non-invasively monitor gene expression by embedding reporters within introns contained in the parent gene.

    • Salima Benbarche
    •  & Omar Abdel-Wahab
    Nature Cell Biology 24, 1571-1573