RNA sequencing

RNA sequencing refers to techniques used to determine the sequence of RNA molecules. It includes high-throughput shotgun sequencing of cDNA molecules obtained by reverse transcription from RNA, and next-generation sequencing technologies to sequence the RNA molecules within a biological sample in an effort to determine the primary sequence and relative abundance of each RNA molecule.

Latest Research and Reviews

News and Comment

  • Research Highlights |

    A recent study combines CRISPR-based perturbation with single-cell RNA sequencing to characterize the roles of epigenome regulator proteins in controlling cell fate and identity during embryonic development.

    • Darren J. Burgess
  • Research Highlights |

    A study in Cell introduces memory sequencing (MemorySeq), a method for identifying genes that are highly and heritably expressed over multiple cell divisions. These expression patterns can reveal cellular subpopulations with distinct phenotypes, such as drug resistance.

    • Dorothy Clyde
  • Research Highlights |

    barRNAseq allows detailed explorations of signaling pathways that control embryonic differentiation.

    • Madhura Mukhopadhyay
    Nature Methods 17, 759
  • Research Highlights |

    A new study in Nature Methods presents the ‘ZipSeq’ spatial transcriptomics approach, whereby patterned illumination is used to print barcodes onto chosen tissue regions.

    • Darren J. Burgess