RNA-binding proteins

RNA-binding proteins are proteins that bind to ribonucleic acid (RNA) molecules, are generally found in the cytoplasm and nucleus, and are important in forming ribonucleoproteins (RNPs). One type of RNP, hnRNPs (heteronuclear proteins), are important in splicing of mRNA (messenger RNA), polyadenylation, stabilization, localization and translation.

Latest Research and Reviews

  • Research | | open

    Low complexity (LC) domains can drive the formation of both amyloid fibrils and protein droplets. Here, the authors identify reversible amyloid cores from the LC of hnRNPA1, based on which they elucidate the structural basis of reversible fibrillation and its interplay with hnRNPA1 droplet formation.

    • Xinrui Gui
    • , Feng Luo
    • , Yichen Li
    • , Heng Zhou
    • , Zhenheng Qin
    • , Zhenying Liu
    • , Jinge Gu
    • , Muyun Xie
    • , Kun Zhao
    • , Bin Dai
    • , Woo Shik Shin
    • , Jianhua He
    • , Lin He
    • , Lin Jiang
    • , Minglei Zhao
    • , Bo Sun
    • , Xueming Li
    • , Cong Liu
    •  & Dan Li
  • Research | | open

    The Staufen family of RNA-binding proteins are conserved microtubule dependent mRNA transporter factors. Here the authors use biochemical and functional approaches to characterize the RNA-binding properties of mouse Staufen 2 and study the mRNA binding capacity of its two domains dsRBDs 1 and 2.

    • Simone Heber
    • , Imre Gáspár
    • , Jan-Niklas Tants
    • , Johannes Günther
    • , Sandra M. Fernandez Moya
    • , Robert Janowski
    • , Anne Ephrussi
    • , Michael Sattler
    •  & Dierk Niessing
  • Research |

    Doudna and colleagues determine the mechanisms used by type V anti-CRISPR proteins. AcrVA1 is a multiple-turnover inhibitor that triggers cleavage of the Cas12a-bound guide RNA, while AcrVA4 and AcrVA5 inhibit recognition of dsDNA.

    • Gavin J. Knott
    • , Brittney W. Thornton
    • , Marco J. Lobba
    • , Jun-Jie Liu
    • , Basem Al-Shayeb
    • , Kyle E. Watters
    •  & Jennifer A. Doudna
  • Research | | open

    RNA binding proteins are important regulators of RNA function. Here the authors describe a method for isolation of RNA-protein complexes that does not rely on a specific RNA sequence or motif, and demonstrate the approach by providing the global RNA-bound proteomes of human HEK293 cells and Salmonella Typhimurium.

    • Erika C. Urdaneta
    • , Carlos H. Vieira-Vieira
    • , Timon Hick
    • , Hans-Herrmann Wessels
    • , Davide Figini
    • , Rebecca Moschall
    • , Jan Medenbach
    • , Uwe Ohler
    • , Sander Granneman
    • , Matthias Selbach
    •  & Benedikt M. Beckmann
  • Research | | open

    Ribosome-associated quality control (RQC) pathways monitor and respond to stalling of the translating ribosome. Here the authors show that the ribosome associated RQC factor Hel2/ZNF598, an E3 ubiquitin ligase, generally interacts with mRNAs in the vicinity of stop codons.

    • Marie-Luise Winz
    • , Lauri Peil
    • , Tomasz W. Turowski
    • , Juri Rappsilber
    •  & David Tollervey

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