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Letter |
Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA
The co-crystal structure of the T-box tRNA-binding region, stem I, bound to tRNA is solved, showing that this region not only binds the anticodon, but also cradles the entire tRNA, forming an extended interface; the two T-loop motifs of stem I mediate interactions similar to those of RNase P and the large ribosomal subunit, even though the three species do not share a common evolutionary ancestor.
- Jinwei Zhang
- & Adrian R. Ferré-D’Amaré
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Letter |
Three-state mechanism couples ligand and temperature sensing in riboswitches
In the human pathogen Vibrio vulnificus, the riboswitch regulating gene expression of the adenosine deaminase is shown to exist in three distinct stable conformational states; this three-state mechanism allows control of gene expression over a broad temperature range, which is essential for Vibrio adaptation.
- Anke Reining
- , Senada Nozinovic
- & Harald Schwalbe
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Letter |
B12 cofactors directly stabilize an mRNA regulatory switch
The crystal structures of two different cobalamin (vitamin B12)-binding riboswitches are determined; the structures reveal how cobalamin facilitates interdomain interactions to regulate gene expression.
- James E. Johnson Jr
- , Francis E. Reyes
- & Robert T. Batey
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Article |
Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch
A riboswitch that binds fluoride was identified recently, which is surprising because both RNA and fluoride are negatively charged; here it is shown that the fluoride ion is coordinated to three positively charged magnesium ions, which are further encased in a negatively charged shell of RNA backbone phosphates and water molecules.
- Aiming Ren
- , Kanagalaghatta R. Rajashankar
- & Dinshaw J. Patel
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Letter |
An equilibrium-dependent retroviral mRNA switch regulates translational recoding
The NMR structure of the murine leukaemia virus recoding translational signal is determined, giving insight into the mechanism of read-through in retroviruses.
- Brian Houck-Loomis
- , Michael A. Durney
- & Victoria M. D’Souza
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Research Highlights |
Synthetic biology: Search and destroy
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Research Highlights |
Biochemistry: Chemical gene switch
Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography
Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA
Three-state mechanism couples ligand and temperature sensing in riboswitches
B12 cofactors directly stabilize an mRNA regulatory switch
Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch
An equilibrium-dependent retroviral mRNA switch regulates translational recoding