Reverse transcription polymerase chain reaction articles from across Nature Portfolio

Reverse transcription polymerase chain reaction (RTPCR) is a technique to detect or quantify RNA. It uses a reverse transcriptase enzyme to convert RNA to DNA, followed by PCR to amplify the DNA.

Latest Research and Reviews

Research
18 December 2022 | Open Access

Defining the timeline of periostin upregulation in cardiac fibrosis following acute myocardial infarction in mice
- Hadas Gil
- , Matan Goldshtein
- & Smadar Cohen
Scientific Reports 12, 21863
Research
09 December 2022 | Open Access

An efficient human stem cells derived cardiotoxicity testing platform for testing oncotherapeutic analogues of quercetin and cinnamic acid
- Saurabh Mandal
- , Naisarg Gamit
- & Sudha Warrier
Scientific Reports 12, 21362
Research
02 December 2022 | Open Access

Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing
- Jin-Mi Oh
- , Minae An
- & Woong-Yang Park
Experimental & Molecular Medicine 54, 2128-2134
Research
18 November 2022 | Open Access

Observation of hapten-induced sensitization responses for the development of a mouse skin sensitization test, including the elicitation phase
- Ryo Kamata
- , Yukiko Okawa
- & Kazuki Takeda
Scientific Reports 12, 19898
Research
02 November 2022 | Open Access

Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh
- Manish Tiwari
- , Monika Sodhi
- & Manishi Mukesh
Scientific Reports 12, 18473
Research
01 November 2022 | Open Access

Reference genes expression stability in Avena sativa L. during compatible and incompatible interactions with Puccinia graminis
- Sylwia Sowa
- , Magdalena Sozoniuk
- & Edyta Paczos-Grzęda
Scientific Reports 12, 18369

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News and Comment

News & Views | 17 June 2022

Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay

We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify CXCL10 mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellular immunity to SARS-CoV-2.

Nature Biotechnology 40, 1584-1585
Research Highlights | 13 August 2021

A pandemic’s trajectory using PCR testing
- Ananya Rastogi
Nature Computational Science 1, 505
Research Highlights | 16 December 2016

Widening the probe
- Grant Miura
Nature Chemical Biology 13, 1
News & Views | 28 August 2015

Removing roadblocks to deep sequencing of modified RNAs

By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.
- Jeremy E Wilusz
Nature Methods 12, 821-822
News & Views | 27 January 2015

Biomarkers in transplantation—the devil is in the detail

Numerous studies have suggested the utility of non-invasive molecular biomarkers to monitor recipients of kidney transplants. A new correlation-based algorithm using stepwise analysis of gene expression data from peripheral blood samples, claiming to detect subclinical, and predict clinical acute allograft rejection, requires corroboration before testing in prospective validation studies.
- Michael Abecassis
- & Bruce Kaplan
Nature Reviews Nephrology 11, 204-205
Research Highlights | 26 November 2013

Cellular hierarchies by single-cell RT-qPCR

Nature Methods 10, 1147

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Reverse transcription polymerase chain reaction articles from across Nature Portfolio

Latest Research and Reviews

Defining the timeline of periostin upregulation in cardiac fibrosis following acute myocardial infarction in mice

An efficient human stem cells derived cardiotoxicity testing platform for testing oncotherapeutic analogues of quercetin and cinnamic acid

Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing

Observation of hapten-induced sensitization responses for the development of a mouse skin sensitization test, including the elicitation phase

Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh

Reference genes expression stability in Avena sativa L. during compatible and incompatible interactions with Puccinia graminis

News and Comment

Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay

A pandemic’s trajectory using PCR testing

Widening the probe

Removing roadblocks to deep sequencing of modified RNAs

Biomarkers in transplantation—the devil is in the detail

Cellular hierarchies by single-cell RT-qPCR

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