Reverse transcription polymerase chain reaction articles from across Nature Portfolio

Here, using CAGE-seq, the authors show that transcription start site choice in thousands of genes may endow transcripts with distinct post-transcriptional fates, with dynamic switching defining cancer cell proliferation, differentiation and treatment response.

We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify CXCL10 mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellular immunity to SARS-CoV-2.

By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.

Numerous studies have suggested the utility of non-invasive molecular biomarkers to monitor recipients of kidney transplants. A new correlation-based algorithm using stepwise analysis of gene expression data from peripheral blood samples, claiming to detect subclinical, and predict clinical acute allograft rejection, requires corroboration before testing in prospective validation studies.