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Coordination of cohesin and DNA replication observed with purified proteins
We study the interplay between cohesin and replication by reconstituting a functional replisome using purified proteins, showing how cohesin initially responds to replication and providing a molecular model for the establishment of sister chromatid cohesion.
- Yasuto Murayama
- , Shizuko Endo
- & Hiroyuki Araki
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Fast and efficient DNA replication with purified human proteins
A biochemical reconstitution of human replisomes that provides a system for future studies of DNA metabolic processes.
- Yasemin Baris
- , Martin R. G. Taylor
- & Joseph T. P. Yeeles
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Article
| Open AccessA conserved mechanism for regulating replisome disassembly in eukaryotes
A conserved mechanism for the regulation of replisome disassembly in eukaryotes is shown using cryo-electron microscopy, revealing a role for DNA in the preservation of replisome integrity.
- Michael Jenkyn-Bedford
- , Morgan L. Jones
- & Tom D. Deegan
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Equilibrium between nascent and parental MCM proteins protects replicating genomes
Mother cells recycle parental MCMs and simultaneously synthesize nascent MCMs, both of which are inherited by daughter cells, in which the former are preferentially used to form active replisomes and the latter adjust the pace of replisome movement to minimize errors during DNA replication.
- Hana Sedlackova
- , Maj-Britt Rask
- & Jiri Lukas
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Letter |
Replisome speed determines the efficiency of the Tus−Ter replication termination barrier
The Tus–Ter termination site of Escherichia coli is not completely efficient in stopping DNA replication, with about half of replisomes bypassing this blockade; here the speed of the replication machinery is shown to determine the outcome of the encounter between the replisome and Tus–Ter.
- Mohamed M. Elshenawy
- , Slobodan Jergic
- & Samir M. Hamdan
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Structure of the eukaryotic MCM complex at 3.8 Å
Cryo-electron microscopy is used to visualize the double hexamer of the eukaryotic minichromosome maintenance complex (MCM), which is assembled during the G1 phase of DNA replication; two interdigitated hexamers have a central channel that tightly fits a DNA duplex, and the orientation of the tilted single hexamers sheds light on many functional aspects, particularly in the initial origin DNA melting.
- Ningning Li
- , Yuanliang Zhai
- & Ning Gao
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Regulated eukaryotic DNA replication origin firing with purified proteins
It has long been a goal to reconstitute eukaryotic DNA replication; here a purified in vitro system from budding yeast containing 16 factors, themselves composed of 42 polypeptides, fulfils the staged process of origin-dependent initiation, including its regulation by kinases.
- Joseph T. P. Yeeles
- , Tom D. Deegan
- & John F. X. Diffley