Proteomic analysis

Definition

Proteomic analysis (proteomics) refers to the systematic identification and quantification of the complete complement of proteins (the proteome) of a biological system (cell, tissue, organ, biological fluid, or organism) at a specific point in time. Mass spectrometry is the technique most often used for proteomic analysis.

Latest Research and Reviews

  • Research |

    A survey of cell envelope proteins and their interacting partners in E. coli by mass spectrometry reveals insights into essential bacterial processes.

    • Mohan Babu
    • , Cedoljub Bundalovic-Torma
    • , Charles Calmettes
    • , Sadhna Phanse
    • , Qingzhou Zhang
    • , Yue Jiang
    • , Zoran Minic
    • , Sunyoung Kim
    • , Jitender Mehla
    • , Alla Gagarinova
    • , Irina Rodionova
    • , Ashwani Kumar
    • , Hongbo Guo
    • , Olga Kagan
    • , Oxana Pogoutse
    • , Hiroyuki Aoki
    • , Viktor Deineko
    • , J Harry Caufield
    • , Erik Holtzapple
    • , Zhongge Zhang
    • , Ake Vastermark
    • , Yogee Pandya
    • , Christine Chieh-lin Lai
    • , Majida El Bakkouri
    • , Yogesh Hooda
    • , Megha Shah
    • , Dan Burnside
    • , Mohsen Hooshyar
    • , James Vlasblom
    • , Sessandra V Rajagopala
    • , Ashkan Golshani
    • , Stefan Wuchty
    • , Jack F Greenblatt
    • , Milton Saier
    • , Peter Uetz
    • , Trevor F Moraes
    • , John Parkinson
    •  & Andrew Emili
  • Research | | open

    SUMO and ubiquitin are key signal transducers in several cellular processes including the DNA-damage response. Here the authors describe a method for selective enrichment of ubiquitin substrates for E3 ligases from complex cellular proteomes and identify the SUMO conjugation machinery as direct RNF4 substrates.

    • Ramesh Kumar
    • , Román González-Prieto
    • , Zhenyu Xiao
    • , Matty Verlaan-de Vries
    •  & Alfred C. O. Vertegaal
  • Research |

    Quantitative mass spectrometry was used to produce a proteomic survey of postnatal human brain regions. Compared to matched RNA-seq, protein levels showed more regional variation, especially for membrane-associated proteins in the neocortex.

    • Becky C. Carlyle
    • , Robert R. Kitchen
    • , Jean E. Kanyo
    • , Edward Z. Voss
    • , Mihovil Pletikos
    • , André M. M. Sousa
    • , TuKiet T. Lam
    • , Mark B. Gerstein
    • , Nenad Sestan
    •  & Angus C. Nairn
    Nature Neuroscience 20, 1787–1795
  • Research |

    The mechanistic basis for the role of the metabolic enzyme BCAA transaminase 1 (BCAT1) in acute myeloid leukaemias.

    • Simon Raffel
    • , Mattia Falcone
    • , Niclas Kneisel
    • , Jenny Hansson
    • , Wei Wang
    • , Christoph Lutz
    • , Lars Bullinger
    • , Gernot Poschet
    • , Yannic Nonnenmacher
    • , Andrea Barnert
    • , Carsten Bahr
    • , Petra Zeisberger
    • , Adriana Przybylla
    • , Markus Sohn
    • , Martje Tönjes
    • , Ayelet Erez
    • , Lital Adler
    • , Patrizia Jensen
    • , Claudia Scholl
    • , Stefan Fröhling
    • , Sibylle Cocciardi
    • , Patrick Wuchter
    • , Christian Thiede
    • , Anne Flörcken
    • , Jörg Westermann
    • , Gerhard Ehninger
    • , Peter Lichter
    • , Karsten Hiller
    • , Rüdiger Hell
    • , Carl Herrmann
    • , Anthony D. Ho
    • , Jeroen Krijgsveld
    • , Bernhard Radlwimmer
    •  & Andreas Trumpp
    Nature 551, 384–388
  • Research | | open

    • Esther Imperlini
    • , Massimiliano Gnecchi
    • , Paola Rognoni
    • , Eduard Sabidò
    • , Maria Chiara Ciuffreda
    • , Giovanni Palladini
    • , Guadalupe Espadas
    • , Francesco Mattia Mancuso
    • , Margherita Bozzola
    • , Giuseppe Malpasso
    • , Veronica Valentini
    • , Giuseppina Palladini
    • , Stefania Orrù
    • , Giovanni Ferraro
    • , Paolo Milani
    • , Stefano Perlini
    • , Francesco Salvatore
    • , Giampaolo Merlini
    •  & Francesca Lavatelli

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