Protein purification articles from across Nature Portfolio

Protein purification is a technique by which a single protein type is isolated from a complex mixture such as a cell lysate. It can refer to purification of a native protein from a biological sample or of a recombinant protein using one of a variety of chemical, physical or biological methods.

Latest Research and Reviews

Research
07 November 2022 | Open Access

Chemically stable fluorescent proteins for advanced microscopy

The engineered hyperfolder YFP (hfYFP) and variants offer unprecedented chemical and thermal stability, making them versatile probes for microscopy as well as for challenging applications like correlative light and electron microscopy and expansion microscopy.
- Benjamin C. Campbell
- , Maria G. Paez-Segala
- & Ce Feng Liu
Nature Methods 19, 1612-1621
Research
26 July 2022 | Open Access

A multifaceted strategy to improve recombinant expression and structural characterisation of a Trypanosoma invariant surface protein
- Hagen Sülzen
- , Jitka Votrubova
- & Sebastian Zoll
Scientific Reports 12, 12706
Research
28 June 2022 | Open Access

SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly

The SpyCatcher-SpyTag system allows protein anchoring and nanoassembly. Here, the authors engineer SpySwitch, a dually switchable Catcher which allows gentle purification of SpyTagged proteins prior to downstream applications such as the assembly of virus-like particles.
- Susan K. Vester
- , Rolle Rahikainen
- & Mark Howarth
Nature Communications 13, 3714
Research
31 May 2022 | Open Access

Substantiation of propitious “Enzybiotic” from two novel bacteriophages isolated from a wastewater treatment plant in Qatar
- Ramya Ramadoss
- , Moza Al-Shukri
- & Annette Shoba Vincent
Scientific Reports 12, 9093
Research
10 November 2021 | Open Access

ASKA technology-based pull-down method reveals a suppressive effect of ASK1 on the inflammatory NOD-RIPK2 pathway in brown adipocytes
- Saki Takayanagi
- , Kengo Watanabe
- & Hidenori Ichijo
Scientific Reports 11, 22009
Research
30 September 2021 | Open Access

A new humanized antibody is effective against pathogenic fungi in vitro
- Tomas Di Mambro
- , Tania Vanzolini
- & Mauro Magnani
Scientific Reports 11, 19500

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News and Comment

News & Views | 07 November 2022

Robust fluorescent proteins for high-resolution microscopy and biochemical techniques

Hyperfolder yellow fluorescent protein (hfYFP) and its variants are fluorescent proteins with high chemical and thermal stability. They resist aggregation, withstand diverse chemical challenges and show promise in expansion and electron microscopies. The chloride resistance and uncanny stability in guanidinium of hfYFP enable fluorescence-guided protein purification under denaturing conditions.

Nature Methods 19, 1534-1535
Research Highlights | 02 July 2020

Design of improved pET expression plasmids
- Arunima Singh
Nature Methods 17, 655
Research Highlights | 29 June 2016

A viral trap for protein interactions

Nature Methods 13, 547
Correspondence | 29 September 2015

Widespread nuclease contamination in commonly used oxygen-scavenging systems
- Gayan Senavirathne
- , Jiaquan Liu
- & Richard Fishel
Nature Methods 12, 901-902

All News & Comment

Protein purification articles from across Nature Portfolio

Latest Research and Reviews

Chemically stable fluorescent proteins for advanced microscopy

A multifaceted strategy to improve recombinant expression and structural characterisation of a Trypanosoma invariant surface protein

SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly

Substantiation of propitious “Enzybiotic” from two novel bacteriophages isolated from a wastewater treatment plant in Qatar

ASKA technology-based pull-down method reveals a suppressive effect of ASK1 on the inflammatory NOD-RIPK2 pathway in brown adipocytes

A new humanized antibody is effective against pathogenic fungi in vitro

News and Comment

Robust fluorescent proteins for high-resolution microscopy and biochemical techniques

Design of improved pET expression plasmids

A viral trap for protein interactions

Widespread nuclease contamination in commonly used oxygen-scavenging systems

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