Protein enrichment articles within Nature Communications

Featured

• Article
  18 March 2023 | Open Access

  PCR-like performance of rapid test with permselective tunable nanotrap

  Lateral flow assays are valuable rapid diagnostic tests, but low sensitivity can hinder their precision. Here, the authors report an enrichment method using nanoporous AAO and red blood cell membranes, which when applied to patient samples prior to analysis can improve sensitivity up to 20-fold.

  • Seong Jun Park
  • , Seungmin Lee
  •  & Jeong Hoon Lee

• Article
  01 September 2021 | Open Access

  Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice

  The in vivo identification of proteins secreted from a specific cell type or tissue remains challenging. Here, the authors develop a proximity labeling-based method to selectively label secreted proteins and combine it with proteomics to identify liver secretory proteins in mouse plasma.

  • Kwang-eun Kim
  • , Isaac Park
  •  & Jae Myoung Suh

• Article
  16 December 2019 | Open Access

  Classification of mouse B cell types using surfaceome proteotype maps

  Analysis of the cell surface proteome (surfaceome) is essential for cell classification but is technically challenging. Here the authors miniaturize and automate the Cell Surface Capture method to increase sensitivity, reproducibility and throughput, and use it to create population-specific surfaceome maps of developing mouse B cells.

  • Marc van Oostrum
  • , Maik Müller
  •  & Bernd Wollscheid

• Article
  27 April 2018 | Open Access

  An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins

  Understanding the functions of protein glycosylation critically depends on methods to efficiently enrich glycoproteins from complex samples. Here, the authors develop a strategy using dendrimer-conjugated benzoboroxole to enhance glycopeptide enrichment, providing the basis for more comprehensive glycoprotein analyses.

  • Haopeng Xiao
  • , Weixuan Chen
  •  & Ronghu Wu

• Article
  06 September 2017 | Open Access

  Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides

  Capture of low-abundance multiply phosphorylated peptides (MPPs) is difficult due to limitation of enrichment materials and their interactions with phosphates. Here the authors show, a smart polymer driven by specific but tunable hydrogen bonding interactions can differentially complex with MPPs, singly phosphorylated and non-modified peptides.

  • Guangyan Qing
  • , Qi Lu
  •  & Taolei Sun