In Brief |
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Brief Communication |
Mapping the 3D orientation of piconewton integrin traction forces
Molecular force microscopy employs a combination of fluorescence polarization microscopy and molecular tension sensors to determine the orientation of cellular forces. The technology is demonstrated for integrin-mediated forces in platelets and fibroblasts.
- Joshua M Brockman
- , Aaron T Blanchard
- & Khalid Salaita
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Tools in Brief |
Instant tracking of single-molecule position and orientation
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Article |
Two-photon polarization microscopy reveals protein structure and function
Membrane protein interactions and conformational changes can be sensitively monitored with two-photon polarization microscopy, a method that takes advantage of the anisotropic absorption properties of fluorescent proteins. The authors applied the method to image G-protein activation and changes in intracellular calcium concentration.
- Josef Lazar
- , Alexey Bondar
- & Stuart J Firestein
