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| Open AccessObservation of a phase transition within the domain walls of ferromagnetic Co3Sn2S2
In the Bloch or Neel domain walls in ferromagnets, the magnetization rotates smoothly from up to down, preserving its magnitude. Here, Lee et al show that Co3Sn2S2 exhibits a phase transition within its domain walls to a state in which the magnetization passes through zero rather than rotating as the wall is traversed.
- Changmin Lee
- , Praveen Vir
- & Joseph Orenstein
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Article
| Open AccessSimultaneous orientation and 3D localization microscopy with a Vortex point spread function
Molecular orientation is often ignored during single-molecule localisation microscopy. Here, the authors use a Vortex point spread function in order to simultaneously estimate the 3D position, dipole orientation and degree of rotational constraint, within 30% of the Cramér-Rao bound limit.
- Christiaan N. Hulleman
- , Rasmus Ø. Thorsen
- & Bernd Rieger
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Article
| Open AccessTurn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
The authors have recently developed molecular force microscopy (MFM) which uses fluorescence polarisation to measure cell-surface receptor force orientation. Here they show that structured illumination microscopes, which inherently use fluorescence polarisation, can be used for MFM in a turn-key manner.
- Aaron Blanchard
- , J. Dale Combs
- & Khalid Salaita
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Article
| Open AccessBirefringent Fourier filtering for single molecule coordinate and height super-resolution imaging with dithering and orientation
Determining the orientation of single molecules in super resolution imaging is challenging. Here, by adding polarization control to phase control in the Fourier plane of the imaging path, parameters such as 3D spatial position, 3D orientation and wobbling or dithering angle can be determined from single molecules.
- Valentina Curcio
- , Luis A. Alemán-Castañeda
- & Miguel A. Alonso
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Article
| Open AccessSuper-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
Polarization microscopy has been combined with single-molecule localization, but it’s often limited in either speed or resolution. Here the authors present polarized Structured Illumination Microscopy (pSIM), a method that uses polarized laser excitation to measure dye orientation during fast super-resolution live cell imaging.
- Karl Zhanghao
- , Xingye Chen
- & Peng Xi
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Article
| Open AccessTrue equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.
- Christophe Jung
- , Peter Bandilla
- & Ulrike Gaul
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Article
| Open AccessDirection of actin flow dictates integrin LFA-1 orientation during leukocyte migration
Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here the authors show that actin flow can orient cell surface integrins during leukocyte migration, suggesting integrin activation by cytoskeletal force.
- Pontus Nordenfelt
- , Travis I. Moore
- & Timothy A. Springer