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| Open AccessCombining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation
Invadosomes degrade extracellular matrix and facilitate cell invasion but their molecular composition is not fully understood. Here, the authors combine laser capture and mass spectrometry to map the proteome of invadosomes, showing that they rely on internal translational activity to maintain their structure.
- Zakaria Ezzoukhry
- , Elodie Henriet
- & Frédéric Saltel
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Article
| Open AccessLymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking
The actomyosin cytoskeleton plays an important role in polarised cell migration. Here the authors identify lymphocyte-specific protein (LSP)-1 as a regulator of actomyosin contractility in macrophages, by competing with supervillin for myosin IIA activators acting specifically on the β-actin isoform.
- Pasquale Cervero
- , Christiane Wiesner
- & Stefan Linder
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| Open AccessActomyosin-dependent dynamic spatial patterns of cytoskeletal components drive mesoscale podosome organization
Podosomes are adhesive cytoskeletal structures found in several cell types, but whether or how they are interconnected is not known. Here the authors demonstrate mesoscale connectivity of podosome clusters by imaging directional flow patterns of podosome components vinculin, talin and F-actin.
- Marjolein B. M. Meddens
- , Elvis Pandzic
- & Alessandra Cambi
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Protrusion force microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes
Podosomes are actin-rich adhesion structures that show periodic oscillations in stiffness. Here, Labernadie et al.develop a method to measure the protrusion force and mechanosensing activity of individual podosomes, using an atomic force microscope and a flexible substrate membrane.
- Anna Labernadie
- , Anaïs Bouissou
- & Isabelle Maridonneau-Parini
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Article
| Open AccessInterplay between myosin IIA-mediated contractility and actin network integrity orchestrates podosome composition and oscillations
Dendritic cells use protrusive structures called podosomes to probe the extracellular environment. Here the authors reveal how interplay between actin-mediated core growth and myosin-mediated tension regulates podosome protrusion.
- K. van den Dries
- , M.B.M Meddens
- & A. Cambi