Origin selection articles within Nature Communications

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  • Article
    | Open Access

    The precise rule of replication origin selection and activation in metazoans remains unclear. Here, the authors identify NFIB as a genome organizer and replication pioneer by facilitating nucleosome remodeling and chromatin assembly of the pre-RC.

    • Wenting Zhang
    • , Yue Wang
    •  & Yongfeng Shang

  • Article
    | Open Access

    A program regulating replication origins ensures the exact duplication of vertebrate genomes. The authors identify a combination of guanine-rich motifs, known to form secondary DNA structures, which are sufficient to assemble efficient replication origins.

    • Jérémy Poulet-Benedetti
    • , Caroline Tonnerre-Doncarli
    •  & Marie-Noëlle Prioleau

  • Article
    | Open Access

    Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions and recruit replicative helicases to form a pre-replication complex independent of the DNA sequence.

    • Sai Li
    • , Michael R. Wasserman
    •  & Shixin Liu

  • Article
    | Open Access

    The range of available copy numbers for cloning vectors is largely restricted to the handful of ORIs that have been isolated from plasmids found in nature. Here the authors introduce a plasmid system that allow for the continuous, finely-tuned control of plasmid copy number between 1 and 800 copies per cell.

    • Miles V. Rouches
    • , Yasu Xu
    •  & Guillaume Lambert

  • Article
    | Open Access

    Replicative hexameric helicases are fundamental components of replisomes. Here the authors resolve a cryo-EM structure of the E1 helicase from papillomavirus bound to a DNA replication fork, providing insights into the mechanism of DNA unwinding by these hexameric enzymes.

    • Abid Javed
    • , Balazs Major
    •  & Elena V. Orlova

  • Article
    | Open Access

    DNA replication processes are often dysregulated in cancer. Here the authors analyse DNA synthesis patterns in cancer cells undergoing partial genome re-replication to reveal that re-replication exhibits aberrant replication fork dynamics and a skewed distribution of replication initiation that over-duplicates early-replicating genomic regions.

    • Haiqing Fu
    • , Christophe E. Redon
    •  & Mirit I. Aladjem

  • Article
    | Open Access

    Eukaryotic DNA replication is regulated to ensure copying of the genome (only) once per cell cycle. Here the authors, using optical trapping and confocal microscopy, demonstrate the dynamics of the origin recognition complex and subsequent intermediates that lead up to the loading of an MCM helicase onto DNA.

    • Humberto Sánchez
    • , Kaley McCluskey
    •  & Nynke H. Dekker

  • Article
    | Open Access

    Contrary to most eukaryotes that lack sequence-specific origins of replication, S. cerevisiae origins are defined by specific DNA sequence motifs. Here the authors reveal that multiple subunits of ORC, including Orc2 and Orc4, contribute to the sequence-specificity of origins in S. cerevisiae.

    • Y. Hu
    • , A. Tareen
    •  & B. Stillman

  • Article
    | Open Access

    DNA replication is initiated at defined genomic sites called origins of replication following ORC pre-replicative complex assembly. Here the authors identify a protein ubiquitylating ORC that is involved in origin activation and may act as a selector of origins to be fired.

    • Philippe Coulombe
    • , Joelle Nassar
    •  & Marcel Méchali

  • Article
    | Open Access

    DNA sequences contribute to the location and timing of replication origin firings. Here by allele-specific analysis, the authors show that replication asynchrony is associated with small cumulative variations in the initiation efficiency of origins, rather than with the activation of dormant origins.

    • Boris Bartholdy
    • , Rituparna Mukhopadhyay
    •  & Eric E. Bouhassira

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