Origin firing articles within Nature Communications

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  • Article
    | Open Access

    Effective and regulated activation of the Mcm2-7 helicase underlies faithful genome replication. Here the authors reveal mechanistic detail how the pre-loading complex proteins TopBP1 and GINS interact and, thus, how the helicase activator GINS loads on Mcm2-7 during replication origin firing.

    • Matthew Day
    • , Bilal Tetik
    •  & Dominik Boos

  • Article
    | Open Access

    Here, the authors show that a DnaA:origin complex promotes specific nucleobase capture from a single DNA strand. It is proposed that this mechanism may play a key role stimulating opening of bacterial chromosome origins.

    • Simone Pelliciari
    • , Salomé Bodet-Lefèvre
    •  & Heath Murray

  • Article
    | Open Access

    Here the authors show that in activated B cells, RIF1 primarily binds early-replicating active chromatin and promotes early replication. RIF1 and MCM proteins establish early replication timing signatures genome-wide and ensure early replication of highly transcribed genes.

    • Daniel Malzl
    • , Mihaela Peycheva
    •  & Rushad Pavri

  • Article
    | Open Access

    Profiling of human DNA polymerase Polε and Polα demonstrates their roles in leading and lagging strand DNA synthesis, and their independent measures allowed accurate predictions of replication dynamics and effects of transcription.

    • Eri Koyanagi
    • , Yoko Kakimoto
    •  & Yasukazu Daigaku

  • Article
    | Open Access

    DNA polymerase epsilon has a critical role in DNA replication initiation. Here, the authors show that in human cancer cells POLE is dispensable for the replicative helicase assembly but not for replication initiation, which requires the non-catalytic domain of POLE1.

    • Sameera Vipat
    • , Dipika Gupta
    •  & Tatiana N. Moiseeva

  • Article
    | Open Access

    The Dbf4-dependent kinase Cdc7 (DDK) is essential for eukaryotic DNA replication. Here, the authors present a series of cryo-EM structures elucidating the versatility of this kinase in exerting an ordered phosphorylation of its essential target to promote replication initiation.

    • Jiaxuan Cheng
    • , Ningning Li
    •  & Yuanliang Zhai

  • Article
    | Open Access

    During pre-Replication Complex, eukaryotic cells load two MCMs into a head-to-head Double Hexamer around duplex DNA (DH). Here the authors preRC assembly assay with purified proteins to reveal insights into S. cerevisiae’s first steps that lead to the DH formation.

    • Marina Guerrero-Puigdevall
    • , Narcis Fernandez-Fuentes
    •  & Jordi Frigola

  • Article
    | Open Access

    DNA replication is initiated at defined genomic sites called origins of replication following ORC pre-replicative complex assembly. Here the authors identify a protein ubiquitylating ORC that is involved in origin activation and may act as a selector of origins to be fired.

    • Philippe Coulombe
    • , Joelle Nassar
    •  & Marcel Méchali

  • Article
    | Open Access

    Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase, which requires the leading-strand polymerase Pol ɛ. Here the authors present a structural analysis of a CMG Pol ɛ on a DNA fork, providing insight on the steps leading productive helicase engagement to the DNA junction.

    • Panchali Goswami
    • , Ferdos Abid Ali
    •  & Alessandro Costa

  • Article
    | Open Access

    The Greatwall/Ensa/PP2A-B55 pathway controls mitotic substrate phosphorylation and mitotic entry. Here the authors show that cells regulate S phase duration by controlling the ubiquitin-proteasome degradation of Treslin in a Gwl/Ensa-dependent pathway.

    • Sophie Charrasse
    • , Aicha Gharbi-Ayachi
    •  & Anna Castro

  • Article
    | Open Access

    The organized initiation of DNA replication at sites throughout the genome must be carefully choreographed to maintain genome stability. Bonne-Andrea and colleagues show that protein SUMOylation controls the density of origin firing, and identify cyclin E as an important substrate in this context.

    • Catherine Bonne-Andrea
    • , Malik Kahli
    •  & Olivier Coux

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