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| Open AccessNuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction
The reported assay shows a subcellular-specific RNA labeling method for efficient enrichment and deep profiling of nuclear and cytoplasmic RBPs, the authors apply this to investigate changes of subcellular-specific RBP-RNA interactions in ferroptosis.
- Haofan Sun
- , Bin Fu
- & Weijie Qin
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| Open AccessNuclear Hsp104 safeguards the dormant translation machinery during quiescence
During aging, proteins are damaged and can misfold, compromising cellular viability. Here, Kohler et al. uncover how aging cells maintain fitness by redirecting the protein repair factor Hsp104 to the nucleus in response to metabolic cues.
- Verena Kohler
- , Andreas Kohler
- & Sabrina Büttner
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| Open AccessDynamical control enables the formation of demixed biomolecular condensates
In this work, the authors report that protein-RNA condensates with shared proteins and distinct RNAs can form and persist in vitro and in cells as distinct entities if the nonshared RNA molecules are dynamically arrested, but the shared protein components are dynamically exchangeable.
- Andrew Z. Lin
- , Kiersten M. Ruff
- & Rohit V. Pappu
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| Open AccessTMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress
Researchers reveal how the stress of the endoplasmic reticulum is transmitted to the nuclear envelope, which swells, and is brought back to physiologic shape by autophagic pathways.
- Marika K. Kucińska
- , Juliette Fedry
- & Maurizio Molinari
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| Open AccessThe coilin N-terminus mediates multivalent interactions between coilin and Nopp140 to form and maintain Cajal bodies
Cajal bodies are membraneless organelles scaffolded by coilin protein. Here, coilin–coilin and coilin–Nopp140 interaction sites are identified and perturbed, revealing coilin’s capacity to form long fibrils and be remodeled into spherical structures.
- Edward Courchaine
- , Sara Gelles-Watnick
- & Karla M. Neugebauer
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| Open AccessCytoplasmic forces functionally reorganize nuclear condensates in oocytes
Cytoskeletal activity generates mechanical forces known to agitate and displace membrane-bound organelles in the cytoplasm. In oocytes, Al Jord et al. discover that these cytoplasmic forces functionally remodel nuclear RNA-processing condensates across scales for developmental success.
- Adel Al Jord
- , Gaëlle Letort
- & Marie-Hélène Verlhac
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| Open AccessNuclear speed and cycle length co-vary with local density during syncytial blastoderm formation in a cricket
Early in insect embryo development, many nuclei share one large cell, travel varied paths and self-organize into a single layer. Donoughe et al. illuminate this process with live-imaging, modeling, and experimental changes to the embryo’s shape.
- Seth Donoughe
- , Jordan Hoffmann
- & Cassandra G. Extavour
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| Open AccessIn situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli
The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome maturation and reveal the native organization of the nucleolus.
- Philipp S. Erdmann
- , Zhen Hou
- & Wolfgang Baumeister
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| Open AccessN-aryl pyrido cyanine derivatives are nuclear and organelle DNA markers for two-photon and super-resolution imaging
It is challenging to develop DNA probes that allow staining of both organelle and nuclear DNA, are compatible with super resolution imaging and avoid UV-light photo-excitation. The authors overcome these issues with N-aryl pyrido cyanine derivatives showing high DNA specificity and membrane permeability.
- Kakishi Uno
- , Nagisa Sugimoto
- & Yoshikatsu Sato
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| Open AccessLocation-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus
The role of mTORC1 at the lysosome is well established, but mTORC1 is known to be active in other cellular locations. Here, the authors develop Akt-STOPS to inhibit Akt specifically in the nucleus and identify a new regulatory mode for mTORC1 distinct in the nucleus.
- Xin Zhou
- , Yanghao Zhong
- & Jin Zhang
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| Open AccessNuclear membrane protein Lem2 regulates nuclear size through membrane flow
Nuclear size scales with cell size, but the underlying mechanisms are unclear. Here, the authors report in fission yeast that the inner nuclear membrane protein Lem2 and the ER membrane protein Lnp1 are barriers to membrane flow and propose that they maintain nuclear size in proportion to cell membrane content.
- Kazunori Kume
- , Helena Cantwell
- & Paul Nurse
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Article
| Open AccessNuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis
The origin and physiological significance of lipid droplets (LDs) in the nucleus is not clear. Here authors show that nucleoplasmic LDs in hepatocytes are derived from apolipoprotein B (ApoB)-free lumenal LDs and constitute a feedback mechanism to regulate PC synthesis in accordance with ER stress.
- Kamil Sołtysik
- , Yuki Ohsaki
- & Toyoshi Fujimoto
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| Open AccessCycles of protein condensation and discharge in nuclear organelles studied by fluorescence lifetime imaging
Studying the condensation of proteins into membraneless organelles in live cells is highly challenging. Here the authors develop a fluorescence lifetime imaging approach to monitor the condensation of proteins in nuclear organelles and report coordinated and cyclic changes in several nuclear organelles.
- Artem Pliss
- , Svitlana M. Levchenko
- & Paras N. Prasad
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| Open AccessExploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.
- Maria J. Sarmento
- , Michele Oneto
- & Luca Lanzanò
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| Open AccessDistinct molecular cues ensure a robust microtubule-dependent nuclear positioning in the Drosophila oocyte
Asymmetric nuclear positioning in the fruit fly oocyte is essential for the correct localization of axis determinants. Here, the authors show that different microtubule-dependent mechanisms contribute to nuclear transport and ensure the robustness of nuclear positioning.
- Nicolas Tissot
- , Jean-Antoine Lepesant
- & Antoine Guichet
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| Open AccessNuclear envelope-associated endosomes deliver surface proteins to the nucleus
Endocytosis typically directs proteins on a recycling route back to the plasma membrane, transport to the Golgi apparatus or delivery to the lysosome. Here Chaumet et al.describe a population of vesicles that can fuse directly with the outer nuclear membrane and deliver cargo into the nuclear envelope, where it can be translocated into the nucleoplasm.
- Alexandre Chaumet
- , Graham D. Wright
- & Frederic Bard
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| Open AccessLoss of lamin A function increases chromatin dynamics in the nuclear interior
Nuclear lamins mediate interactions between chromatin and the nuclear envelope, however they are also found throughout the nucleoplasm. By measuring the dynamics of different genomic loci, Bronshtein et al.show that lamin A is also required for the stability of the nuclear interior.
- I. Bronshtein
- , E. Kepten
- & Y. Garini
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| Open AccessThe tethering of chromatin to the nuclear envelope supports nuclear mechanics
The mechanical properties of the metazoan nucleus can be influenced by the nuclear lamina. Here, Schreiner et al.show that untethering chromatin from the inner nuclear membrane results in highly deformable, softer nuclei, revealing an important role for chromatin in modulating nuclear mechanics.
- Sarah M. Schreiner
- , Peter K. Koo
- & Megan C. King
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| Open AccessDendritic geometry shapes neuronal cAMP signalling to the nucleus
Neurons have complex dendritic trees but the rules governing the propagation of signals from dendrites to nuclei remain unclear. Here the authors combine diffusion-reaction modelling and live imaging to investigate the mechanisms regulating cAMP signalling in neurons and find that dendritic tree geometry shapes synapse-to-nucleus signalling.
- Lu Li
- , Nicolas Gervasi
- & Jean-Antoine Girault
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Chromatin decompaction by the nucleosomal binding protein HMGN5 impairs nuclear sturdiness
Whether heterochromatin affects the physical properties of the nucleus is not known. Here, Furusawa et al. show that chromatin decompaction decreases the sturdiness of the nucleus in cultured cells and leads to lamina disruption and cardiac abnormalities in adult mice, suggesting a structural, non-genetic function for heterochromatin.
- Takashi Furusawa
- , Mark Rochman
- & Michael Bustin