Nucleotide-binding proteins articles from across Nature Portfolio

Here the authors use cryogenic electron microscopy to solve the Arabidopsis thaliana Argonaute10–guide RNA complex with and without a target RNA representing a slicing substrate, to provide insights into the slicing mechanism of a eukaryotic Argonaute.

Cryo-EM of human PRPS1 shows the nucleotide-synthesizing enzyme assembling into filaments that accommodate active and inhibited conformations. Engineered and disease mutations reveal that filament assembly stabilizes allosteric sites, enhancing catalytic activity.

SARM1 is a key player in axon degeneration. Here, the authors generate a nanobody, which specifically recognizes the NMN-bound state of SARM1 and helps resolve the SARM1 structure in an intermediate state of activation.

The structure of the complete dynein–dynactin complex and its interaction with microtubules and cargo adaptors are visualized using cryo-electron microscopy.

Through structural analysis of the activation of bacterial STING, the molecular basis of STING filament formation and TIR effector domain activation in antiphage signalling is defined.