Nucleolus

  • Article
    | Open Access

    rDNA repeats residing in the nucleolus must be released to the nucleoplasm to allow repair by homologous recombination. Here the authors reveal insights into the molecular mechanism proposing that phosphorylation and SUMOylation of the rDNA-tethering complex facilitate the nucleolar release of damaged repeats to maintain genome integrity.

    • Matías Capella
    • , Imke K. Mandemaker
    •  & Sigurd Braun
  • Article
    | Open Access

    Many RNA viruses that replicate in the cytoplasm express proteins that localize to nucleoli, but the nucleolar functions remain largely unknown. Here, the authors show that the Henipavirus matrix protein mimics an endogenous Treacle partner of the DNA-damage response, resulting in suppression of rRNA biogenesis.

    • Stephen M. Rawlinson
    • , Tianyue Zhao
    •  & Gregory W. Moseley
  • Article
    | Open Access

    Cytidine deaminase (CDA) deficiency leads to genome instability. Here the authors find a synthetic lethal interaction between CDA and the microtubule-associated protein Tau deficiencies, and report that Tau depletion affects rRNA synthesis, ribonucleotide pool balance, and rDNA stability.

    • Elias Bou Samra
    • , Géraldine Buhagiar-Labarchède
    •  & Mounira Amor-Guéret
  • Article
    | Open Access

    Animal lifespan is plastic and is regulated by conserved signalling pathways. Here, Tikuet al.show that longevity-enhancing mutations or interventions are associated with reduced nucleolar size in worms, flies, mice and humans, and that nucleolar size can predict life-expectancy in individual worms.

    • Varnesh Tiku
    • , Chirag Jain
    •  & Adam Antebi
  • Article
    | Open Access

    HGPS is a premature aging disease caused by mutations in the nuclear protein lamin A. Here, the authors show that cells from patients with HGPS have expanded nucleoli and increased protein synthesis, and report that nucleoli also expand as aging progresses in cells derived from healthy individuals.

    • Abigail Buchwalter
    •  & Martin W. Hetzer
  • Article
    | Open Access

    Nucleoplasmic translocation of NPM1 is integral to nucleolar stress sensing. Here, the authors show that nucleolar oxidation is a general cellular stress response, and that oxidation-related glutathionylation of NPM1 triggers its translocation and facilitates p53 activation.

    • Kai Yang
    • , Ming Wang
    •  & Jing Yi
  • Article
    | Open Access

    The nucleolus is a specialized functional domain of the nucleus where ribosome biogenesis is initiated and also implicated in a p53-dependent anti-tumor surveillance. Here the authors use a quantitative imaging approach to detail the role of each ribosomal protein on the structural integrity of the nucleolus and p53 homeostasis.

    • Emilien Nicolas
    • , Pascaline Parisot
    •  & Denis L. J. Lafontaine