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| Open AccessNucleolar reorganization after cellular stress is orchestrated by SMN shuttling between nuclear compartments
DNA damage causes a major reorganization of the nucleolus. Here, the authors find that this structural restoration depends on the shuttling of the protein SMN from the Cajal bodies to the nucleolus, which requires coilin and PRMT1.
- Shaqraa Musawi
- , Lise-Marie Donnio
- & Giuseppina Giglia-Mari
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Article
| Open AccessMapping nucleolus-associated chromatin interactions using nucleolus Hi-C reveals pattern of heterochromatin interactions
Here the authors developed a nucleolus Hi-C technique (nHi-C) for enriching nucleolus-associated interactions, and revealed specific heterochromatin interaction patterns within and around nucleoli in human cells at high resolution.
- Ting Peng
- , Yingping Hou
- & Cheng Li
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Article
| Open AccessPQBP5/NOL10 maintains and anchors the nucleolus under physiological and osmotic stress conditions
The nucleolus is a liquid-liquid phase separation droplet that dynamically changes under stress. Here, the authors report PQBP5/NOL10 is a critical component of the nucleolus, maintaining its structure and position in the nucleus during osmotic stress.
- Xiaocen Jin
- , Hikari Tanaka
- & Hitoshi Okazawa
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Article
| Open AccessRG/RGG repeats in the C. elegans homologs of Nucleolin and GAR1 contribute to sub-nucleolar phase separation
Spaulding et al. survey RG/RGG repeats in C. elegans and identify the homologs of Nucleolin (NUCL-1) and GAR1 (GARR-1). RG/RGG repeats are dispensable for nucleolar accumulation but critical for sub-nucleolar phase separation.
- Emily L. Spaulding
- , Alexis M. Feidler
- & Dustin L. Updike
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Article
| Open AccessGenome-wide maps of nucleolus interactions reveal distinct layers of repressive chromatin domains
Mapping nucleolar-associated domains (NADs) is challenging as the nucleolus is membrane-less. Here the authors adapted the DNA adenine methyltransferase identification (DamID) method by engineering a “nucleolar histone” that can mark genomic contacts with the nucleolus through DNA methylation (m6A) and characterized NADs in mouse embryonic stem cells (ESCs) and derived neural progenitors (NPCs).
- Cristiana Bersaglieri
- , Jelena Kresoja-Rakic
- & Raffaella Santoro
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Article
| Open AccessNucleolar release of rDNA repeats for repair involves SUMO-mediated untethering by the Cdc48/p97 segregase
rDNA repeats residing in the nucleolus must be released to the nucleoplasm to allow repair by homologous recombination. Here the authors reveal insights into the molecular mechanism proposing that phosphorylation and SUMOylation of the rDNA-tethering complex facilitate the nucleolar release of damaged repeats to maintain genome integrity.
- Matías Capella
- , Imke K. Mandemaker
- & Sigurd Braun
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Article
| Open AccessIdentification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
Ribosome synthesis is a complex multi-step process. Here the authors present a method that allows the efficient isolation and characterization of the preribosomal complexes formed along the entire ribosome synthesis pathway in human cells.
- Blanca Nieto
- , Sonia G. Gaspar
- & Mercedes Dosil
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Article
| Open AccessViral regulation of host cell biology by hijacking of the nucleolar DNA-damage response
Many RNA viruses that replicate in the cytoplasm express proteins that localize to nucleoli, but the nucleolar functions remain largely unknown. Here, the authors show that the Henipavirus matrix protein mimics an endogenous Treacle partner of the DNA-damage response, resulting in suppression of rRNA biogenesis.
- Stephen M. Rawlinson
- , Tianyue Zhao
- & Gregory W. Moseley
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Article
| Open AccessA role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival
Cytidine deaminase (CDA) deficiency leads to genome instability. Here the authors find a synthetic lethal interaction between CDA and the microtubule-associated protein Tau deficiencies, and report that Tau depletion affects rRNA synthesis, ribonucleotide pool balance, and rDNA stability.
- Elias Bou Samra
- , Géraldine Buhagiar-Labarchède
- & Mounira Amor-Guéret
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Article
| Open AccessSmall nucleoli are a cellular hallmark of longevity
Animal lifespan is plastic and is regulated by conserved signalling pathways. Here, Tikuet al.show that longevity-enhancing mutations or interventions are associated with reduced nucleolar size in worms, flies, mice and humans, and that nucleolar size can predict life-expectancy in individual worms.
- Varnesh Tiku
- , Chirag Jain
- & Adam Antebi
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Article
| Open AccessNucleolar expansion and elevated protein translation in premature aging
HGPS is a premature aging disease caused by mutations in the nuclear protein lamin A. Here, the authors show that cells from patients with HGPS have expanded nucleoli and increased protein synthesis, and report that nucleoli also expand as aging progresses in cells derived from healthy individuals.
- Abigail Buchwalter
- & Martin W. Hetzer
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Article
| Open AccessA redox mechanism underlying nucleolar stress sensing by nucleophosmin
Nucleoplasmic translocation of NPM1 is integral to nucleolar stress sensing. Here, the authors show that nucleolar oxidation is a general cellular stress response, and that oxidation-related glutathionylation of NPM1 triggers its translocation and facilitates p53 activation.
- Kai Yang
- , Ming Wang
- & Jing Yi
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Article
| Open AccessInvolvement of human ribosomal proteins in nucleolar structure and p53-dependent nucleolar stress
The nucleolus is a specialized functional domain of the nucleus where ribosome biogenesis is initiated and also implicated in a p53-dependent anti-tumor surveillance. Here the authors use a quantitative imaging approach to detail the role of each ribosomal protein on the structural integrity of the nucleolus and p53 homeostasis.
- Emilien Nicolas
- , Pascaline Parisot
- & Denis L. J. Lafontaine