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| Open AccessRNA export through the nuclear pore complex is directional
There is little information on export directionality of RNA passing through the nuclear pore complex in human cells. Here, the authors examine single RNA molecules in transit, to demonstrate 5’-first directionality for the export of mRNA and lncRNA.
- Asaf Ashkenazy-Titelman
- , Mohammad Khaled Atrash
- & Yaron Shav-Tal
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| Open AccessCo-translational assembly orchestrates competing biogenesis pathways
The biogenesis of nuclear pores imposes a logistic challenge for cells. Here, the authors investigate structural motifs for co-translational interactions in nucleoporins and find that co-translational assembly events differ between paralogous assembly pathways thus contributing to faithful assembly.
- Maximilian Seidel
- , Anja Becker
- & Martin Beck
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| Open AccessA nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.
- Sarah A. Nordeen
- , Kasper R. Andersen
- & Thomas U. Schwartz
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| Open AccessYeast Nup84-Nup133 complex structure details flexibility and reveals conservation of the membrane anchoring ALPS motif
The Y complex is an essential component of the nuclear pore complex but a full model based on experimental structures is lacking. Here, the authors complete the model of the yeast Y complex with two nanobody-bound crystal structures, providing molecular insights into its flexibility and membrane anchoring.
- Sarah A. Nordeen
- , Daniel L. Turman
- & Thomas U. Schwartz
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| Open AccessNucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores
The contribution of central and peripheral channels of nuclear pores to transport of transmembrane proteins is unclear. Here the authors show that most inner nuclear membrane proteins use only peripheral channels, but some extend nuclear localization signals into the central channel for directed nuclear transport.
- Krishna C. Mudumbi
- , Rafal Czapiewski
- & Weidong Yang
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| Open AccessNucleoporin Nup155 is part of the p53 network in liver cancer
The nuclear pore complex (NPC) is known to regulate p53 signaling and this has mainly been linked to peripheral NPC subunits. Here the authors show that Nup155 from the NPC inner ring regulates the p53 pathway by controlling p21 translation while also being a target of p53-mediated repression.
- Kerstin Holzer
- , Alessandro Ori
- & Stephan Singer
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| Open AccessThe path of pre-ribosomes through the nuclear pore complex revealed by electron tomography
Large protein complexes and ribonucleoprotein particles (RNPs) such as pre-ribosomes are transported from the nucleus to the cytoplasm through the nuclear pore complex (NPC). Here the authors use ultrafast freezing and electron tomography to catch snapshots of native RNPs crossing the NPC and estimate their transit time using a probabilistic model.
- Franck Delavoie
- , Vanessa Soldan
- & Pierre-Emmanuel Gleizes
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| Open AccessIn situ architecture of the algal nuclear pore complex
While the architecture of vertebrate nuclear pore complexes (NPCs) is well understood, the extent of its evolutionary conservation is still unclear. Here, the authors analyze the in situ architecture of an algal NPC, revealing distinct structural features that provide insights into NPC evolution.
- Shyamal Mosalaganti
- , Jan Kosinski
- & Martin Beck
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Article
| Open AccessStructural and functional analysis of mRNA export regulation by the nuclear pore complex
The export of mRNA to the cytosol depends on the nuclear pore complex (NPC) and the activation of the helicase DDX19, but their interplay in humans remains poorly understood. Here, the authors present a structural and functional analysis of DDX19 activation, revealing how the human NPC regulates mRNA export.
- Daniel H. Lin
- , Ana R. Correia
- & André Hoelz
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| Open AccessA SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits
The nuclear pore complex is crucial for mediating nucleocytoplasmic exchanges. Here the authors use budding yeast to reveal a mechanism responsible of maintaining nucleoporin homeostasis by sensing changes in the complex integrity and further altering the metabolism of the corresponding mRNAs.
- Jérôme O. Rouvière
- , Manuel Bulfoni
- & Benoit Palancade
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Article
| Open AccessSystematic analysis of protein turnover in primary cells
The proteome-wide characterization of proteostasis depends on robust approaches to determine protein half-lives. Here, the authors improve the accuracy and precision of mass spectrometry-based quantification, enabling reliable protein half-life determination in several non-dividing cell types.
- Toby Mathieson
- , Holger Franken
- & Mikhail M. Savitski
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Article
| Open AccessCombining Spinach-tagged RNA and gene localization to image gene expression in live yeast
Measuring single-cell mRNA dynamics is critical to understand gene expression. Here, using RNA Spinach technique to detect very low abundant mRNAs, Guet et al. report an analysis of the osmotic shock response in live yeast by localizing induced transcription factors, target gene loci and corresponding transcripts.
- David Guet
- , Laura T. Burns
- & Catherine Dargemont
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Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process
Damaged DNA is often targeted to nuclear pore complexes for repair. Here, the authors show that kinesin-14 mediates this process ensuring error-prone repair, while perinuclear telomere attachment licenses damaged telomeric loci for this repair and kinesin-14 blocks senescence in the absence of telomerase.
- Daniel K.C. Chung
- , Janet N.Y. Chan
- & Karim Mekhail
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Article
| Open AccessStructure and gating of the nuclear pore complex
Nuclear pore complexes (NPCs) are large macromolecular assemblies that mediate the exchange of molecules between the nucleus and cytoplasm. Here the authors present a ∼20 Å cryo-EM structure of the X. laevisNPC in different states of transport to propose a model for the architecture of the NPC’s molecular gate within its central channel.
- Matthias Eibauer
- , Mauro Pellanda
- & Ohad Medalia
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| Open AccessChromatin organization at the nuclear pore favours HIV replication
Retroviruses such as HIV integrate into the host genome as an essential step prior to their replication. Here Lelek et al. identify nuclear pore complex proteins that are essential for HIV nuclear import and productive integration, and show that the intranuclear protein Tpr influences integration into transcriptionally active chromatin.
- Mickaël Lelek
- , Nicoletta Casartelli
- & Francesca Di Nunzio
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| Open AccessCytoplasmic TAF2–TAF8–TAF10 complex provides evidence for nuclear holo–TFIID assembly from preformed submodules
TFIID is an essential transcription factor complex that controls the expression of most protein-coding genes in eukaryotes. Here the authors identify and characterize a complex containing TAF2, TAF8 and TAF10, which assembles in the cytoplasm before integration into the nuclear holo–TFIID complex.
- Simon Trowitzsch
- , Cristina Viola
- & Imre Berger