NMR spectroscopy

Nuclear magnetic resonance (NMR) spectroscopy is a technique that detects the chemical environment of atomic nuclei by the absorption of radio-frequency electromagnetic radiation when in the presence of a high magnetic field. NMR is used in chemistry and related fields for high-resolution molecular structure determination and the study of molecular dynamics.

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  • News and Views |

    An NMR method to monitor conformational states of challenging large protein targets is described. The method, which can be used to evaluate distances between two labels and to measure conformational exchange rates, revealed an unanticipated outward-facing state in a glutamate transporter.

    • Ricky C. Cheng
    •  & Merritt Maduke
  • News and Views |

    Cellular protein levels are finely tuned through microRNA-mediated gene regulation, triggered by RNA-induced silencing complexes (RISCs) that recognize, bind and silence mRNA targets. A recent study shows that, while mRNA target recognition is achieved with only about a third of the guide RNA sequence, formation of an efficient RISC conformation required for target silencing involves the coordination of transient structural states of the entire guide RNA–mRNA duplex.

    • François Major
  • Research Highlights |

    A newly developed method allows structure elucidation of proteins inside intact mammalian cells, using solid-state NMR spectroscopy.

    • Arunima Singh
    Nature Methods 16, 676
  • News and Views |

    A strategy using pressure was devised to structurally identify conformational transitions in protein ensembles, allowing the rational prediction of mutations that induce pressure-driven enzyme activation. These results highlight the power of flexibility–function analyses in protein engineering design and applications.

    • Nicolas Doucet
    Nature Catalysis 2, 646-647
  • Research Highlights |

    Analysis of complex mixtures often requires time consuming separation techniques. Mathias Nilsson and co-workers have, now, developed a new approach to dissecting the NMR spectra of unaltered complex mixtures.

    • Gabriella Graziano