Neurochemistry

  • Article
    | Open Access

    α-ketoglutarate (αKG) is an intermediate in the tricarboxylic acid cycle that is required in the nucleus for genomic DNA demethylation by Tet3. Here, the authors show that the enzyme glutamate dehydrogenase, which converts glutamate to αKG, is redirected from the mitochondria to the nucleus.

    • Franziska R. Traube
    • , Dilara Özdemir
    •  & Thomas Carell
  • Article
    | Open Access

    Several mechanisms contribute to the maintenance of constant extracellular pH, essential for normal brain function. Here the authors show that astrocytes help to control local brain pH via a neuronal activity-dependent release of bicarbonate by the electrogenic sodium bicarbonate cotransporter 1.

    • Shefeeq M. Theparambil
    • , Patrick S. Hosford
    •  & Alexander V. Gourine
  • Article
    | Open Access

    CRISPR-guided cytidine deaminases, including BE3 (Base Editor 3) and Target-AID (activation-induced cytidine deaminase), can covert C:G base pairs to T:A at target site. Here, the authors generate missense mutations of mouse Psen1 gene and find BE3 has higher editing efficiency than Target-AID.

    • Hiroki Sasaguri
    • , Kenichi Nagata
    •  & Takaomi C. Saido
  • Article
    | Open Access

    The Parkinson’s disease‐associated LRRK2 protein is a multidomain Roco protein with GTPase activity. Here the authors use a multidisciplinary approach to characterize the GTPase mechanism of a homologous bacterial Roco protein and give mechanistic insights into disease-causing LRRK2 mutations.

    • Egon Deyaert
    • , Lina Wauters
    •  & Wim Versées
  • Article
    | Open Access

    Imaging cellular activity in mouse spinal cord has been historically difficult. Here the authors develop cellular resolution fluorescence imaging approaches in the spinal cord of behaving mice, and report distinct activity patterns of neurons and astrocytes in response to different sensory inputs.

    • Kohei J. Sekiguchi
    • , Pavel Shekhtmeyster
    •  & Axel Nimmerjahn
  • Article
    | Open Access

    Hypothalamic neurons expressing melanin-concentrating-hormone (MCH) maintain body weight by orchestrating behaviour and metabolism, but little is known about their intrinsic regulation. Here, Gonzalez and colleagues reveal their behaviour-related dynamics during wakefulness, and map their brain-wide neural inputs.

    • J. Antonio González
    • , Panagiota Iordanidou
    •  & Denis Burdakov
  • Article
    | Open Access

    The neural mechanisms underlying efficient coding of natural sensory stimuli have yet to be fully determined. Here, monitoring sensory pyramidal cells in weakly electric fish, the authors find SK channels are necessary for matching the responsiveness of neurons to natural stimuli and subsequent behavioural responses.

    • Chengjie G. Huang
    • , Zhubo D. Zhang
    •  & Maurice J. Chacron
  • Article
    | Open Access

    Food anticipation is thought to be initiated by the central clock in the brain. Here the authors show that the peripheral organs initiate this process by showing that liver-specific deletion of Per2 can inhibit food anticipation by interfering with ß-hydroxybutyrate production and its subsequent processing in the brain.

    • Rohit Chavan
    • , Céline Feillet
    •  & Urs Albrecht