Nanopores articles from across Nature Portfolio

Nanopores are nanoscale pores in electrically insulating materials used to study the physical properties of biomolecules by measuring changes in current as individual molecules transit. Nanopore arrays are constructed from channel proteins in lipid membranes or from pores patterned in synthetic materials, and can sequence DNA or characterize protein folding.

Latest Research and Reviews

Research
20 March 2023 | Open Access

A generalizable nanopore sensor for highly specific protein detection at single-molecule precision

Sensitive and accurate approaches for protein detection have many potential applications. Here the authors show how engineered protein nanopore sensors, consisting of a monobody fused to a single-polypeptide nanopore, can be used for highly specific detection of proteins in complex biofluids.
- Mohammad Ahmad
- , Jeung-Hoi Ha
- & Liviu Movileanu
Nature Communications 14, 1374
Research
23 February 2023 | Open Access

High durability and stability of 2D nanofluidic devices for long-term single-molecule sensing
- Mukeshchand Thakur
- , Nianduo Cai
- & Aleksandra Radenovic
npj 2D Materials and Applications 7, 11
Research | 20 February 2023

Protein nanopore reveals the renin–angiotensin system crosstalk with single-amino-acid resolution

A nanopore framework has been developed to reveal the crosstalk effect on the renin–angiotensin system. By reading the single-amino-acid differences in angiotensin peptides with high accuracy and high efficiency, the selective inhibition of angiotensin-converting enzyme by angiotensin-converting enzyme 2 was revealed. This activity was shown to be suppressed by the spike protein of SARS-CoV-2.
- Jie Jiang
- , Meng-Yin Li
- & Yi-Tao Long
Nature Chemistry, 1-9
Research
16 January 2023 | Open Access

Simultaneous identification of viruses and viral variants with programmable DNA nanobait

Polymerase-chain-reaction-based methods used in clinic to identify respiratory viral infections cannot simultaneously detect multiple viruses or viral variants. Here nanopore nucleic acid sensing is coupled to programmable ribonuclease to simultaneously identify specific short RNA sequences of multiple viruses, without predetection steps such as sample purification or preamplification.
- Filip Bošković
- , Jinbo Zhu
- & Ulrich F. Keyser
Nature Nanotechnology 18, 290-298
Research
10 January 2023 | Open Access

Improved mineralization of dental enamel by electrokinetic delivery of F⁻ and Ca²⁺ ions
- NamBeng Tay
- , HiongYap Gan
- & Jongyoon Han
Scientific Reports 13, 516
Research | 09 January 2023

Unidirectional single-file transport of full-length proteins through a nanopore

Full-length, unfolded proteins are slowly translocated through nanopores without enzymes and fingerprinted.
- Luning Yu
- , Xinqi Kang
- & Meni Wanunu
Nature Biotechnology, 1-10

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News and Comment

Editorial | 10 March 2023

Single-cell proteomics: challenges and prospects

Single-cell proteomics is a challenging goal and an area of rapid methods development. This Focus issue highlights the many paths toward high-throughput, high-sensitivity measurements.

Nature Methods 20, 317-318
Comments & Opinion | 06 October 2022

Advances in nanopore direct RNA sequencing

Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early adopters have used this technology to document nucleotide modifications and 3′ polyadenosine tails on RNA strands without added chemistry steps. Individual strands ranging in length from 70 to 26,000 nucleotides have been sequenced. In our opinion, broader acceptance of nanopore DRS by molecular biologists and cell biologists will be accelerated by higher basecall accuracy and lower RNA input requirements.
- Miten Jain
- , Robin Abu-Shumays
- & Mark Akeson
Nature Methods 19, 1160-1164
Research Highlights | 06 September 2022

Nanopores distinguish RNA modifications

Different nucleotides and derivatives can be read out by MspA-PBA, an engineered nanopore.
- Lin Tang
Nature Methods 19, 1035
News & Views | 02 September 2022

Detection of modified RNA with an engineered nanopore

MspA engineered nanopore allows for high-resolution detection of modifications on RNA.
- Sukanya Punthambaker
Nature Nanotechnology 17, 1044-1045
News & Views | 25 April 2022

Harnessing blue energy with COF membranes

Anion-selective covalent organic framework (COF) membranes fabricated using laminar assembly and interfacial polymerization exhibit enhanced potential for osmotic power generation.
- Laxmicharan Samineni
- & Manish Kumar
Nature Nanotechnology 17, 564-566
Research Highlights | 11 January 2022

Reading proteins with nanopores

Two studies use nanopores for single-protein fingerprinting and make headway toward single-protein sequencing.
- Rita Strack
Nature Methods 19, 31

All News & Comment

Nanopores articles from across Nature Portfolio

Latest Research and Reviews

A generalizable nanopore sensor for highly specific protein detection at single-molecule precision

High durability and stability of 2D nanofluidic devices for long-term single-molecule sensing

Protein nanopore reveals the renin–angiotensin system crosstalk with single-amino-acid resolution

Simultaneous identification of viruses and viral variants with programmable DNA nanobait

Improved mineralization of dental enamel by electrokinetic delivery of F⁻ and Ca²⁺ ions

Unidirectional single-file transport of full-length proteins through a nanopore

News and Comment

Single-cell proteomics: challenges and prospects

Advances in nanopore direct RNA sequencing

Nanopores distinguish RNA modifications

Detection of modified RNA with an engineered nanopore

Harnessing blue energy with COF membranes

Reading proteins with nanopores

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