Nanocrystallography

This study documents equine ACE2 (eqACE2) binding to the RBDs of SARS-CoV-2 and related CoVs, revealing a mechanism of eqACE2 binding with RaTG13-RBD, SARS-CoV-2 prototype-RBD and Omicron BA.1-RBD.

DNA N6-adenine methylation (6 mA) plays a crucial role in epigenetic regulation in eukaryotes. Here, the authors determined nine crystal structures of the ciliates 6 mA methyltransferase complexes, providing the molecular basis for understanding the functions of 6 mA.

Activation of the Tn7 transposase depends on the Tn7-encoded AAA+ ATPase adaptor, TnsC. A new cryo-EM structure reveals how TnsC targets DNA sequences between target and insertion sites to restrict insertion events to control sequence-specific transposon insertion.

Ayan et al. report two structures of the protein streptavidin - one at ambient temperature determined using serial femtosecond crystallography and a second one determined at cryogenic temperature. These results provide insights into the structural dynamics of apo streptavidin and reveal a cooperative allostery between monomers for binding to biotin, and the findings are supported by GNM analysis.