Nanobiotechnology

  • Article
    | Open Access

    Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.

    • Shanwen Zhang
    • , Qian Ren
    •  & Huan Bao
  • Article
    | Open Access

    The concentration of a biomarker in solution can be determined by counting single molecules. Here the authors report a digital immunoassay scheme with solid-state nanopore readout to quantify a target protein and use this to measure thyroid-stimulating hormone from human serum.

    • Liqun He
    • , Daniel R. Tessier
    •  & Vincent Tabard-Cossa
  • Article
    | Open Access

    Cell death is a critical process in health and disease, yet available markers record later stages of cell death once a cell has already begun to decompose. Here the authors show the use of a genetically encoded calcium indicator that demarcates an irreversible stage of cell death earlier than previously possible.

    • Jeremy W. Linsley
    • , Kevan Shah
    •  & Steven Finkbeiner
  • Article
    | Open Access

    Brain organoids derived from human pluripotent stem cells can model human brain development and disease, though current culture systems fail to ensure reliable production of high-quality organoids. Here the authors combine human brain extracellular matrix and culture in a microfluidic device to promote structural and functional maturation of human brain organoids.

    • Ann-Na Cho
    • , Yoonhee Jin
    •  & Seung-Woo Cho
  • Article
    | Open Access

    The authors have recently developed molecular force microscopy (MFM) which uses fluorescence polarisation to measure cell-surface receptor force orientation. Here they show that structured illumination microscopes, which inherently use fluorescence polarisation, can be used for MFM in a turn-key manner.

    • Aaron Blanchard
    • , J. Dale Combs
    •  & Khalid Salaita
  • Article
    | Open Access

    Placenta accreta spectrum (PAS) is a high-risk obstetrical complication associated with significant morbidity and mortality. Here the authors discover a uniquely high prevalence of circulating trophoblasts clusters in PAS and explore their diagnostic potential to augment current diagnostic modalities for the early detection of PAS.

    • Yalda Afshar
    • , Jiantong Dong
    •  & Yazhen Zhu
  • Article
    | Open Access

    Self-assembling peptides have a range of potential applications but developing self-assembling sequences can be challenging. Here, the authors report on a one-bead one-compound combinatorial library where fluorescence is used to detect the potential for self-assembly and identified candidates are evaluated.

    • Pei-Pei Yang
    • , Yi-Jing Li
    •  & Kit S. Lam
  • Article
    | Open Access

    Self-powered implantable devices have the potential to extend device operation, though current energy harvesters are both insufficient and inconvenient. Here the authors report on a commercial coin battery-sized high-performance inertia-driven triboelectric nanogenerator based on body motion and gravity that can be used to charge a lithium-ion battery and integrated into a cardiac pacemaker.

    • Hanjun Ryu
    • , Hyun-moon Park
    •  & Sang-Woo Kim
  • Article
    | Open Access

    Nucleic acid-based constitutional dynamic networks (CDNs) enable control of various catalytic processes, but it is challenging to achieve intercommunication between different CDNs and by that mimic complex cell biology networks. Here, the authors report two CDNs that control the integration of photochemical and dark-operating processes, and show their intercommunication afforded by environmental components.

    • Chen Wang
    • , Michael P. O’Hagan
    •  & Itamar Willner
  • Article
    | Open Access

    Limited understanding of the interactions between nanoparticle drug carriers and the blood-brain barrier underlies many translational failures in treatments of brain disorders. Here the authors use two-photon microscopy in mice to characterize the receptor-mediated transcytosis of nanoparticles at all steps of delivery from the blood to the brain in vivo.

    • Krzysztof Kucharz
    • , Kasper Kristensen
    •  & Martin Johannes Lauritzen
  • Review Article
    | Open Access

    This review presents an overview of scenarios where van der Waals (vdW) materials provide unique advantages for nanophotonic biosensing applications. The authors discuss basic sensing principles based on vdW materials, advantages of the reduced dimensionality as well as technological challenges.

    • Sang-Hyun Oh
    • , Hatice Altug
    •  & Michael S. Strano
  • Article
    | Open Access

    Precise and dynamic manipulation of nano-objects on a large scale has been challenging. Here, the authors introduce acoustoelectronic nanotweezers, combining precision of electronic tweezers with large-field dynamic control of acoustic tweezers, demonstrating complex patterning of sub-100 nm objects.

    • Peiran Zhang
    • , Joseph Rufo
    •  & Tony Jun Huang
  • Article
    | Open Access

    Organising proteins in 2D and 3D is needed to develop complex bimolecular materials for a range of applications. Here, the authors report the encapsulation of ferritin and apoferritin in DNA-based voxels with programmed assembly to generate both 2D and 3D protein lattices and demonstrate the retention of protein function.

    • Shih-Ting Wang
    • , Brian Minevich
    •  & Oleg Gang
  • Article
    | Open Access

    Halide perovskite has been applied for resistive switching memory devices, but there are challenges remained to achieve practical application. By using high-throughput screening based on first-principles calculations, the authors discover that lead-free dimer-Cs3Sb2I9 meets the requirements, which exhibits switching speed of 20 ns.

    • Youngjun Park
    • , Seong Hun Kim
    •  & Jang-Sik Lee
  • Article
    | Open Access

    miRNA profiling from patient blood can be used for cancer diagnosis. Here the authors present an electro-optical nanopore sensing platform which allows sensitive and specific miRNA detection directly in human serum and apply to monitoring of miR-141-3p and miR-375-3p in different stage of prostate cancer.

    • Shenglin Cai
    • , Thomas Pataillot-Meakin
    •  & Joshua B. Edel
  • Article
    | Open Access

    The effect of fallopian tube’s curvature on sperm motion has not been studied in detail. Here, the authors use droplet microfluidics to create soft curved interfaces, revealing a dynamic switch in sperm motility from a progressive surface-aligned mode at low curvatures, to an aggressive surface-attacking mode at high curvatures.

    • Mohammad Reza Raveshi
    • , Melati S. Abdul Halim
    •  & Reza Nosrati
  • Article
    | Open Access

    SERS assays have potential for multiplexed detection of biomarkers but differentiation of SERS tags remains a challenge. Here, the authors report the creation of 14 distinct geometrically controlled metal carbonyl tags and demonstrate multiplexed detection of nasopharyngeal carcinoma biomarkers from patient blood.

    • Duo Lin
    • , Chang-Lin Hsieh
    •  & Kien Voon Kong
  • Article
    | Open Access

    Dysfunction of the lymphatic system leads to secondary lymphedema and results in degradation of quality of life. Here, the authors show that delivery of nucleoside-modified Vascular Endothelial Growth Factor C (VEGFC) mRNA, encapsulated in lipid nanoparticles, induces organ-specific lymphatic growth and reverses experimental lymphedema.

    • Dániel Szőke
    • , Gábor Kovács
    •  & Zoltán Jakus
  • Article
    | Open Access

    Gold nanoparticles (Au NPs) with enzyme-like activities are useful glucose oxidase mimics, but the insights into the mechanism of this reaction are limited. Here, the authors show that the process of glucose oxidation by Au NPs is analogous to the one catalysed by glucose oxidase, involving dehydrogenation and oxygen reduction to H2O2; and that other noble metal NPs also catalyse glucose dehydrogenation, but oxygen is preferably reduced to water.

    • Jinxing Chen
    • , Qian Ma
    •  & Shaojun Dong
  • Article
    | Open Access

    Nanopores have been used for direct observation of RNA structure in native environments but have limited RNA differentiation capabilities. Here, the authors report on the use of Mycobacterium smegmatis porin A nanopores for the trapping and translocation identification of microRNA, siRNA, tRNA and ribosomal RNA.

    • Yuqin Wang
    • , Xiaoyu Guan
    •  & Shuo Huang
  • Article
    | Open Access

    Dielectric metasurfaces have different Q-factor and light localisation requirements for sensing and imaging. Here, the authors present a dielectric metasurface, supporting two optical modes with sharp Fano resonances for high Q-factors and strong spatial confinement, allowing both sensing and imaging.

    • Donato Conteduca
    • , Isabel Barth
    •  & Thomas F. Krauss
  • Article
    | Open Access

    A major challenge across a variety of fields is how to process the vast quantities of data produced by sensors without large computation resources. Here, the authors present a neuromorphic chip which can detect a relevant signature of epileptogenic tissue from intracranial recordings in patients.

    • Mohammadali Sharifshazileh
    • , Karla Burelo
    •  & Giacomo Indiveri
  • Article
    | Open Access

    In-situ methods are important for investigating the local structure and function in molecular nanostructures but such investigations often involve laborious labeling methods that can disrupt behavior or are not fast enough to capture stimuli-responsive phenomena. Here, the authors use X-rays resonant with molecular bonds to demonstrate an in-situ nanoprobe that eliminates the need for labels and enables data collection times within seconds.

    • Terry McAfee
    • , Thomas Ferron
    •  & Brian A. Collins
  • Article
    | Open Access

    Molecular transport across physical barriers requires pores that connect to the environment. Here, the authors report on a solution based dynamic study into the effects of size and charge on the transport through the pores of virus-like particles derived from bacteriophage P22 using an encapsulated redox system.

    • Ekaterina Selivanovitch
    • , Benjamin LaFrance
    •  & Trevor Douglas
  • Article
    | Open Access

    Small interfering RNA (siRNA) is used to regulate gene expression for therapeutic purposes, but the design of stable and efficient siRNA delivery systems is challenging. Here, the authors develop a siRNA-encapsulated and aptamer-incorporated core/shell nanoparticle for controlled siRNA delivery, with high stability, tumor-specific targeting and long circulation time.

    • Chang Xue
    • , Shuyao Hu
    •  & Zai-Sheng Wu
  • Article
    | Open Access

    Relatively little is known about cell-matrix interactions and the intracellular transduction of an initial ligand-receptor binding event on the single-molecule level. Here authors combine ligand-decorated DNA tension sensors with DNA-PAINT super-resolution microscopy to study the mechanical engagement of single integrin receptors and the downstream influence on actin bundling.

    • Thomas Schlichthaerle
    • , Caroline Lindner
    •  & Ralf Jungmann
  • Article
    | Open Access

    Investigating biomembrane curvature formation is important for studying intracellular processes, but the instability of liposome models mimicking these membranes restricts exploration of membrane processes. Here, the authors demonstrate control over the curvature formation in polymersome membranes by insertion of PNIPAm as stimuli responsive polymer.

    • Jiawei Sun
    • , Sjoerd J. Rijpkema
    •  & Daniela A. Wilson
  • Article
    | Open Access

    Stabilizing lipid nanoparticles and lipidprotein assemblies is challenging owing to lipid dynamics and protein instability. Here, the authors report on the reversible encapsulation of liposomes and proteoliposomes in a metalorganic framework permitting months-long room temp storage.

    • Fabian C. Herbert
    • , Sameera S. Abeyrathna
    •  & Jeremiah J. Gassensmith
  • Article
    | Open Access

    Reovirus endocytosis depends on junctional adhesion molecule A (JAM-A) and β1 integrin binding. Here, Koehler et al. use single-virus force spectroscopy and confocal microscopy to demonstrate a direct interaction between reovirus and β1 integrins via viral capsid protein λ2, which promotes clathrin recruitment to cell-bound reovirus.

    • Melanie Koehler
    • , Simon J. L. Petitjean
    •  & David Alsteens
  • Article
    | Open Access

    Outer membrane vesicles (OMVs), non-replicative particles secreted by Gram-negative bacteria, are known for their immunostimulatory and adjuvant properties. Here, by employing a Plug-and-Display technology, the authors engineer OMVs to display tumor antigens on the surface, a platform that promotes anti-tumor immune responses in preclinical cancer models.

    • Keman Cheng
    • , Ruifang Zhao
    •  & Guangjun Nie
  • Article
    | Open Access

    The immunostimulatory properties of TLR7/8 agonists, such as resiquimod, have been exploited for cancer immunotherapy. Here, the authors design platelet membrane-cloaked nanoparticles for selective intratumoral delivery of resiquimod, resulting in potent anti-tumor immune response in a range of preclinical solid tumors.

    • Baharak Bahmani
    • , Hua Gong
    •  & Jie Zhang
  • Article
    | Open Access

    For programmable DNA self-assembly, it is desirable to suppress spontaneous nucleation to enable all-or-nothing assembly of nanostructures far larger than a single DNA origami. Here the authors introduce crisscross polymerization of elongated slat monomers that engage beyond nearest neighbors, providing strictly seed-initiated nucleation of crisscross ribbons with distinct widths and twists.

    • Dionis Minev
    • , Christopher M. Wintersinger
    •  & William M. Shih
  • Article
    | Open Access

    Here, the authors present photonic resonator interferometric scattering microscopy, which utilises a dielectric photonic crystal as the sample substrate. The resonant near-field enhancement leads to improved signal to noise ratio without increasing illumination intensity.

    • Nantao Li
    • , Taylor D. Canady
    •  & Brian T. Cunningham
  • Article
    | Open Access

    There is a clinical need to monitor immune-related toxicities of immune checkpoint blockade therapy. Here, the authors develop a digital SERS platform for multiplexed single cytokine counting to track immune-toxicities and demonstrate the ability to use pre-screening to identify patients at higher risk.

    • Junrong Li
    • , Alain Wuethrich
    •  & Matt Trau
  • Article
    | Open Access

    Single-molecule fluorescence currently requires specialized imaging equipment due to the low signal of a single emitter. Here the authors introduce NanoAntennas with Cleared HOtSpots (NACHOS) to boost the signal sufficient for detection of a single emitter by a smartphone, opening the door to point-of-care applications.

    • Kateryna Trofymchuk
    • , Viktorija Glembockyte
    •  & Philip Tinnefeld
  • Article
    | Open Access

    Coiled-coil protein origami is a strategy for the de novo design of polypeptide nanostructures based on coiled-coil dimer forming peptides, where a single chain protein folds into a polyhedral cage. Here, the authors design a single-chain triangular bipyramid and also demonstrate that the bipyramid can be self-assembled as a heterodimeric complex, comprising pre-defined subunits.

    • Fabio Lapenta
    • , Jana Aupič
    •  & Roman Jerala
  • Article
    | Open Access

    Carbon dots have attracted much attention for biomedical applications but potential degradation and associated toxicity are still poorly understood. Here, the authors report on a study into the photo-degradation of carbon dots, the products produced and associated cytotoxicity.

    • Yue-Yue Liu
    • , Nan-Yang Yu
    •  & Ai-Jun Miao
  • Article
    | Open Access

    Nanozymes have been used for antibacterial applications but have potential toxicity to mammalian cells. Here the authors suggest that nanozymes that generate surface bound reactive oxygen species disrupt bacterial cell walls but not mammalian cells walls due to the different particle uptake mechanisms.

    • Feng Gao
    • , Tianyi Shao
    •  & Lihua Yang
  • Article
    | Open Access

    Polymeric nanocarriers used for drug delivery are often coated by albumin in vivo influencing the therapeutic outcomes. Here, the authors engineer the surface chemistry of a nanocarrier to either stabilize or denature the structure of adsorbed albumin, resulting in evasion or clearance by macrophages.

    • Michael P. Vincent
    • , Sharan Bobbala
    •  & Evan A. Scott
  • Article
    | Open Access

    Understanding the biomolecular corona is of key importance to nanomedicine. Here, the authors report on cryo-electron and tomographic imaging of the corona formed on model nanoparticles and the 3D reconstruction of the corona to study the distribution and association of the biomolecules with the nanoparticle.

    • Sara Sheibani
    • , Kaustuv Basu
    •  & Morteza Mahmoudi