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| Open AccessLongevity interventions modulate mechanotransduction and extracellular matrix homeostasis in C. elegans
Mechanotransduction can be defined as translating physical forces into gene expression, which subsequently drives cell fate. Here, Teuscher et al. showed that mechanotransduction across multiple tissues and extracellular matrices is essential for promoting longevity in vivo.
- Alina C. Teuscher
- , Cyril Statzer
- & Collin Y. Ewald
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| Open AccessMaintenance of appropriate size scaling of the C. elegans pharynx by YAP-1
Imbalance in the growth rate of two organs can perturb their appropriate scaling. Here, Stojanovski et al., identify a mechanism involving the mechanotransducer YAP-1 which ensures proper proportions of the pharynx and the body length of C. elegans.
- Klement Stojanovski
- , Ioana Gheorghe
- & Benjamin D. Towbin
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| Open AccessA pharmacoproteomic landscape of organotypic intervention responses in Gram-negative sepsis
Sepsis can cause organ damage through disparate immunological and metabolic processes. Here the authors demonstrate a proteomics-based scoring strategy for quantifying quantitative and organotypic changes in relationship to dosing, timing, and potential synergistic intervention combinations during sepsis.
- Tirthankar Mohanty
- , Christofer A. Q. Karlsson
- & Johan Malmström
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| Open AccessNucleocytoplasmic transport of active HER2 causes fractional escape from the DCIS-like state
HER2 receptor aberrations are more common in breast DCIS premalignancy than in breast cancer. Here the authors identify a feedback circuit involving HER2 nucleocytoplasmic transport that may explain why some DCIS lesions progress and others do not.
- Lixin Wang
- , B. Bishal Paudel
- & Kevin A. Janes
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| Open AccessJag1-Notch cis-interaction determines cell fate segregation in pancreatic development
Notch signaling is crucial for pancreatic cell fate choice. With mathematical modeling and experiments, Xu et al. provides new insights into how different Notch ligands and Hes1 oscillation guide the spatial-temporal dynamics of cell differentiation.
- Xiaochan Xu
- , Philip Allan Seymour
- & Palle Serup
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| Open AccessSlowest possible replicative life at frigid temperatures for yeast
It is unclear what constraints exist on cellular life in frigid environments. Here, the authors demonstrate that reactive oxygen species and gene-expression speed impose a barrier to replication at low temperatures in yeast, with lower levels enabling quicker replication, and develop a model to describe this phenomenon.
- Diederik S. Laman Trip
- , Théo Maire
- & Hyun Youk
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Article
| Open AccessHigh resolution microfluidic assay and probabilistic modeling reveal cooperation between T cells in tumor killing
Anti-cancer cytotoxic T cell responses largely vary among individuals. Here authors show, by stochastic modeling on high throughput T cell behavior and matched tumor spheroid fate data generated by a microfluidics system, that tumor killing is dependent on T cell cooperativity, which might contribute to the heterogeneity of T cell responses.
- Gustave Ronteix
- , Shreyansh Jain
- & Charles N. Baroud
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| Open AccessPluripotency factors determine gene expression repertoire at zygotic genome activation
Zygotic genome activation in zebrafish relies on pluripotency transcription factors Pou5f3 and Sox19b. Here the authors investigate how these factors interact in vivo by analyzing the changes in chromatin state and time-resolved transcription in Pou5f3 and Sox19b single and double mutant embryos.
- Meijiang Gao
- , Marina Veil
- & Daria Onichtchouk
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| Open AccessSingle-cell analysis identifies a key role for Hhip in murine coronal suture development
Craniofacial development depends on formation and maintenance of sutures between bones of the skull. Here the authors identify enriched expression of the hedgehog inhibitor Hhip, specifically in the mesenchyme of the murine coronal suture, and show sutural dysgenesis in Hhip−/− mutants.
- Greg Holmes
- , Ana S. Gonzalez-Reiche
- & Ethylin Wang Jabs
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| Open AccessLive cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
Spatial transcriptomics aims to pair omic data with tissue structure. Here the authors report Spatially PhotoActivatable Colour Encoded Cell Address Tags (SPACECAT) to track and isolate live cells by location; this enables spatially informed downstream assays like scRNA-seq and flow cytometry.
- Alex S Genshaft
- , Carly G. K. Ziegler
- & Alex K. Shalek
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Article
| Open AccessC. elegans colony formation as a condensation phenomenon
Phase separation phenomena have emerged as being of critical importance in biology. Here, using colony formation in C. elegans as model, the authors demonstrate that the basic concepts that underpin phase separation at a molecular level also apply to collective phenomena at the level of a population of organisms.
- Yuping Chen
- & James E. Ferrell Jr.
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Article
| Open AccessSynthetic neural-like computing in microbial consortia for pattern recognition
Complex biological systems have individual cells acting collectively to solve complex tasks. Here the authors implement neural network-like computing in a bacterial consortia to recognise patterns.
- Ximing Li
- , Luna Rizik
- & Ramez Daniel
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Article
| Open AccessThe singularity response reveals entrainment properties of the plant circadian clock
Phase response curves reveal how biological clocks respond to stimuli applied during different circadian phases but can be costly to produce. Here Masuda et al. show that phase response curves for plants can be reconstructed by monitoring how a desynchronized population responds to a single stimulus.
- Kosaku Masuda
- , Isao T. Tokuda
- & Hirokazu Fukuda
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| Open AccessAutomated design of synthetic microbial communities
In naturally occurring microbial systems, there is a positive relationship between species diversity and productivity of the community. Here the authors perform model selection to find potential amensal interactions that yield robust stable synthetic microbial consortia.
- Behzad D. Karkaria
- , Alex J. H. Fedorec
- & Chris P. Barnes
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| Open AccessMechanical forces drive ordered patterning of hair cells in the mammalian inner ear
The periodic organization of cells is typically associated with mechanisms based on intercellular signaling such as lateral inhibition and Turing patterning. Here the authors show that hair cells in the inner ear rearrange gradually into a checkerboard-like pattern through a tissue-wide shear motion that coordinates intercalation and delamination events.
- Roie Cohen
- , Liat Amir-Zilberstein
- & David Sprinzak
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| Open AccessPredicting cell-to-cell communication networks using NATMI
Single cell expression data allows for inferring cell-cell communication between cells expressing ligands and those expressing their cognate receptors. Here the authors present an updated and curated database of ligand-receptor pairs and a Python-based toolkit to construct and analyse communication networks from single cell and bulk expression data.
- Rui Hou
- , Elena Denisenko
- & Alistair R. R. Forrest
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| Open AccessChemotactic behaviour of Escherichia coli at high cell density
Swimming bacteria perform collective motion at high cell density, yet it is unclear how this behaviour affects their ability to follow substance gradients in the environment. Here, Colin et al. address this question by studying motion of Escherichia coli in controlled chemical gradients.
- Remy Colin
- , Knut Drescher
- & Victor Sourjik
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| Open AccessDynamic intercellular transport modulates the spatial patterning of differentiation during early neural commitment
How heterogeneities arise in stem cell populations remains unclear. Here, Glen et al. find that in ESC colonies cell cycle asynchronies modulate gap junctions, causing variation in intracellular signalling molecule diffusion between cells, and ultimately in spatial heterogeneity in differentiation.
- Chad M. Glen
- , Todd C. McDevitt
- & Melissa L. Kemp
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| Open AccessIridophores as a source of robustness in zebrafish stripes and variability in Danio patterns
Iridophores interact with other cells to give zebrafish their stripes, but what drives their form shifts is unknown. Here, modelling allows the authors to identify robust cues that may specify iridophore form and to find changes to these cues that likely account for altered patterns on related fish.
- Alexandria Volkening
- & Björn Sandstede