Molecular biology articles from across Nature Portfolio

We conducted a comprehensive long-read RNA sequencing (RNA-seq) benchmarking experiment by combining spike-ins and in silico mixtures to establish a ground-truth dataset. We used long- and short-read RNA-seq technology to deeply sequence samples and compared the performance of a range of analysis tools on these data.

We developed CellOT, a tool that integrates optimal transport with input convex neural networks to predict molecular responses of individual cells to various perturbations. By learning a map between the unpaired distributions of unperturbed and perturbed cells, CellOT outperforms current methods and generalizes the inference of treatment outcomes in unobserved cell types and patients.

In organisms with X and Y chromosomes, gene expression must be equalized between the sexes. A protein that causes upregulation of gene expression of the X chromosome in male mosquitoes has been discovered.

Although the catalogue of human protein-coding genes is nearing completion, the number of non-coding RNA genes remains highly uncertain, and for all genes much work remains to be done to understand their functions.

CRISPR enzymes require a defined protospacer adjacent motif (PAM) which can be limiting for editing applications. Here the authors recombine the PAM-interacting domain of SpRY with the N-terminus of Sc + + to generate a chimeric enzyme with highly flexible PAM preference: SpRYc.

We conducted a comprehensive long-read RNA sequencing (RNA-seq) benchmarking experiment by combining spike-ins and in silico mixtures to establish a ground-truth dataset. We used long- and short-read RNA-seq technology to deeply sequence samples and compared the performance of a range of analysis tools on these data.

We developed CellOT, a tool that integrates optimal transport with input convex neural networks to predict molecular responses of individual cells to various perturbations. By learning a map between the unpaired distributions of unperturbed and perturbed cells, CellOT outperforms current methods and generalizes the inference of treatment outcomes in unobserved cell types and patients.

In organisms with X and Y chromosomes, gene expression must be equalized between the sexes. A protein that causes upregulation of gene expression of the X chromosome in male mosquitoes has been discovered.