Microscopy articles from across Nature Portfolio

To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.

We introduce GelMap, a flexible workflow for reporting deformations and anisotropy in expansion microscopy. By intrinsically calibrating the expansion hydrogel using a fluorescent grid that scales with expansion and deforms with anisotropy, GelMap enables the reliable quantification of expansion factors and correction of deformations.

The LED-integrated excitation cube (LEC) is compact, low-cost, and easy to install, taking full advantage of the benefits of LED excitations, and can be used in any fluorescent microscope.

The mechanical and thermal properties of semicrystalline polymers are governed by the hierarchical structure comprising lamellar crystals, but the tilt angles of the molecular chains in the lamellae and their origin remains controversial. Here, the authors report a direct determination of molecular chain orientation in the lamellar crystals of high-density polyethylene using electron-diffraction based imaging with nanometre-scale positional resolution.

The time correlation between primary electrons and the secondary particles in electron microscopies encloses information of the whole interaction and excitation dynamics. The authors introduce time-correlated electron and photon counting microscopy that acts as a universal analysis approach for photon generation processes and arbitrary emitters.

The optimized and disordered structures of planar diffractive lenses enable sub-diffraction limit focusing but destroy wide-field imaging. Here, the authors introduce the information entropy to evaluate the disorder of PDL and achieve good balance between super-focusing and imaging.

The GelMap workflow adds a fluorescent grid into samples before expansion, allowing for precise determination of expansion factor and subsequent deformation correction in ExM. GelMap works with diverse samples and expansion methods.

Montage parallel array cryo-tomography adopts principles of montage tomography via regular array beam-image-shift montage acquisition and is robust for imaging large fields of view while retaining high-resolution structural information in cryo-electron tomography.

To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.

We introduce GelMap, a flexible workflow for reporting deformations and anisotropy in expansion microscopy. By intrinsically calibrating the expansion hydrogel using a fluorescent grid that scales with expansion and deforms with anisotropy, GelMap enables the reliable quantification of expansion factors and correction of deformations.

The LED-integrated excitation cube (LEC) is compact, low-cost, and easy to install, taking full advantage of the benefits of LED excitations, and can be used in any fluorescent microscope.

An expansion microscopy technique called ChromExM offers detailed views into the organization chromatin and associated gene expression machinery in embryos.

The introduction of a two-step deconvolution workflow maximizes the detection of fluorescence in fluctuation-based super-resolution imaging, enabling a square millimetre field of view to be captured in as little as ten minutes.