Microscopy articles within Nature Methods

Featured

  • Research Highlight |

    A microscope objective inspired by the Schmidt telescope offers a large field of view, high numerical aperture, long working distance and compatibility with all homogeneous immersion media for versatile bioimaging.

    • Rita Strack
  • Research Briefing |

    Photoselective sequencing is a new method for genomic and epigenomic profiling within specific regions of a biological specimen that are chosen using light microscopy. This combination of spatial and sequencing information preserves the connections between genomic and environmental properties and deepens our understanding of structure–function relationships in cells and tissues.

  • Comment |

    Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning grassroots community effort is fast building the profile and revealing the impact of vEM technology in the life sciences and clinical research.

    • Lucy M. Collinson
    • , Carles Bosch
    •  & Paul Verkade
  • Research Highlight |

    A new twist on expansion microscopy called Magnify uses a mechanically sturdy gel to simultaneously anchor and expand diverse biological samples for super-resolution imaging.

    • Rita Strack
  • Method to Watch |

    Advances in fluorescence microscopy and spectroscopy show their promise for applications that complement in situ structural biology methods like cryoelectron tomography.

    • Rita Strack
  • Research Briefing |

    Localization Model Fit (LocMoFit) is a tool that enables fitting of super-resolution microscopy data to an arbitrary geometric model. The fit extracts quantitative parameters of individual cellular structures, which can be used to investigate dynamic and heterogenous protein assemblies and to create average protein distribution maps.

  • Article
    | Open Access

    Localization Model Fit (LocMoFit) is an open-source tool for extracting meaningful parameters from individual structures in localization microscopy data. The framework was used for quantitative analysis of diverse biological structures.

    • Yu-Le Wu
    • , Philipp Hoess
    •  & Jonas Ries
  • Article |

    The parallel cryo electron tomography (PACE-tomo) method increases the throughput on in situ samples by parallelizing acquisition. It maximizes the usable sample area on individual lamellae without compromising data quality.

    • Fabian Eisenstein
    • , Haruaki Yanagisawa
    •  & Radostin Danev
  • Research Briefing |

    Hyperfolder yellow fluorescent protein (hfYFP) and its variants are fluorescent proteins with high chemical and thermal stability. They resist aggregation, withstand diverse chemical challenges and show promise in expansion and electron microscopies. The chloride resistance and uncanny stability in guanidinium of hfYFP enable fluorescence-guided protein purification under denaturing conditions.

  • Article
    | Open Access

    The engineered hyperfolder YFP (hfYFP) and variants offer unprecedented chemical and thermal stability, making them versatile probes for microscopy as well as for challenging applications like correlative light and electron microscopy and expansion microscopy.

    • Benjamin C. Campbell
    • , Maria G. Paez-Segala
    •  & Ce Feng Liu
  • Editorial |

    This month we continue our yearly celebration of the beauty of imaging.

  • Research Briefing |

    A combination of light-sheet fluorescence microscopy (LSFM) with structured illumination doubles resolving power over LSFM alone. We show a practical implementation using a single objective for illumination and fluorescence detection and demonstrate its use for rapid volumetric imaging.

  • News & Views |

    The generation of a whole larval zebrafish brain electron microscopy volume in tandem with automated tools lays the groundwork for producing the first vertebrate brain connectome.

    • Paul Brooks
    • , Andrew Champion
    •  & Marta Costa
  • Research Briefing |

    Light-Seq combines high resolution imaging with next generation sequencing of selected cell populations in fixed biological samples. Specifically, microscopically analyzed cells can be subjected to RNA expression profiling while keeping the sample intact for further assays, enabling cellular phenotypes and states to be assessed in the context of the original tissue.

  • News & Views |

    Advances in microscopy, computer vision and open source software are converging to usher in a new era of microscopes that control themselves.

    • Henry Pinkard
    •  & Laura Waller
  • Article
    | Open Access

    Event-triggered STED is an automated approach that can initiate 2D or 3D STED imaging of specific regions in biological samples after detection of an event of interest. This approach can help maximize observations in live cell imaging and enable discovery.

    • Jonatan Alvelid
    • , Martina Damenti
    •  & Ilaria Testa
  • This Month |

    Your name is on the door to your new lab. Life is getting exciting and turbulent.

    • Vivien Marx
  • Brief Communication
    | Open Access

    A systematic exploration of MINFLUX nanoscopy with DNA-PAINT labeling leads to improved nanoscopy in fixed cells and MINFLUX imaging with increased multiplexing, as exemplified by three-color imaging of mitochondria in mammalian cells.

    • Lynn M. Ostersehlt
    • , Daniel C. Jans
    •  & Stefan Jakobs
  • Research Highlight |

    Deep Visual Proteomics combines the power of deep-learning-based image analysis with microdissection and ultrasensitive mass spectrometry to provide insights into the spatial proteome.

    • Rita Strack
  • Research Highlight |

    A newly described fluorescent protein, StayGold, is bright and extremely photostable, enabling extended time-lapse imaging.

    • Rita Strack
  • Technology Feature |

    Widespread adoption of new embryo models hinges on how faithfully they mirror real embryos.

    • Vivien Marx
  • Article
    | Open Access

    A dynamic model of the 4Pi point spread function enables localization microscopy with exceptional three-dimensional resolution and a simpler optical design. 4Pi-STORM images of neurons and mitochondria reveal new details of nanoscale protein and nucleic acid organization.

    • Mark Bates
    • , Jan Keller-Findeisen
    •  & Stefan W. Hell
  • News & Views |

    A flexible open-top light-sheet microscope has been developed that can perform deep three-dimensional imaging on all clearing protocols with low and high optical resolution.

    • Shigeaki Kanatani
    •  & Per Uhlén