Methylation analysis

Methylation analysis is the study of chromosomal patterns of DNA or histone modification by methyl groups. The cytosine (C) base in DNA and lysine residue in histone tails can be methylated. Methylation constitutes an epigenetic mark because its location can affect the regulation of gene expression in a heritable fashion.

Latest Research and Reviews

Research | 23 November 2020

Simultaneous profiling of chromatin accessibility and methylation on human cell lines with nanopore sequencing

Using nanopore sequencing as readout, nanoNOMe–seq enables chromosome-level allele-specific profiles of CpG methylation and chromatin accessibility on human cells, in which the chromatin accessibility is profiled through exogenous GpC methylation introduced by a GpC methyltransferase.
- Isac Lee
- , Roham Razaghi
- , Timothy Gilpatrick
- , Michael Molnar
- , Ariel Gershman
- , Norah Sadowski
- , Fritz J. Sedlazeck
- , Kasper D. Hansen
- , Jared T. Simpson
- & Winston Timp
Nature Methods 17, 1191-1199
Research
20 November 2020 | Open Access

Modular affinity-labeling of the cytosine demethylation base elements in DNA
- Fanny Wang
- , Osama K. Zahid
- , Uday Ghanty
- , Rahul M. Kohli
- & Adam R. Hall
Scientific Reports 10, 20253
Research
25 August 2020 | Open Access

Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
- M. Konki
- , N. Lindgren
- , M. Kyläniemi
- , R. Venho
- , E. Laajala
- , B. Ghimire
- , R. Lahesmaa
- , J. Kaprio
- , J. O. Rinne
- & R. J. Lund
Scientific Reports 10, 14192
Research
24 July 2020 | Open Access

Structural equation modelling analysis determining causal role among methyltransferases, methylation, and apoptosis during human pregnancy and abortion
- Nishat Fatima
- , Syed Habeeb Ahmed
- , S. S. Chauhan
- , Owais Mohammad
- & Syed Mohd. Fazlur Rehman
Scientific Reports 10, 12408
Research
22 July 2020 | Open Access

DNA methylation of the INSR gene as a mediator of the association between prenatal exposure to famine and adulthood waist circumference
- Zhenghe Wang
- , Jieyun Song
- , Changwei Li
- , Yanhui Li
- , Luqi Shen
- , Bin Dong
- , Zhiyong Zou
- & Jun Ma
Scientific Reports 10, 12212
Research
09 July 2020 | Open Access

Comprehensive characterization of claudin-low breast tumors reflects the impact of the cell-of-origin on cancer evolution

Claudin-low tumors are a rare aggressive subtype of breast cancers. In this study, the authors use a multiomics approach to demonstrate that these tumors are heterogeneous and comprise three main subgroups that emerge from different evolutionary processes.
- Roxane M. Pommier
- , Amélien Sanlaville
- , Laurie Tonon
- , Janice Kielbassa
- , Emilie Thomas
- , Anthony Ferrari
- , Anne-Sophie Sertier
- , Frédéric Hollande
- , Pierre Martinez
- , Agnès Tissier
- , Anne-Pierre Morel
- , Maria Ouzounova
- & Alain Puisieux
Nature Communications 11, 3431

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News and Comment

Research Highlights | 20 July 2020

Closing in on cfDNA-based detection and diagnosis

Two papers in Nature Medicine report studies that validate the utility of cell-free methylated DNA analysis for early detection of renal cell carcinomas and diagnosis of central nervous system tumours.
- Sarah Seton-Rogers
Nature Reviews Cancer 20, 481
Correspondence
16 April 2019 | Open Access

Reprogrammable CRISPR/dCas9-based recruitment of DNMT1 for site-specific DNA demethylation and gene regulation
- Anrui Lu
- , Jingman Wang
- , Weihong Sun
- , Weiren Huang
- , Zhiming Cai
- , Guoping Zhao
- & Jin Wang
Cell Discovery 5, 22
Research Highlights | 28 March 2019

Bisulfite-free epigenetic sequencing
- Lei Tang
Nature Methods 16, 286
Research Highlights | 30 January 2019

Detecting changes in methylation landscape
- Lei Tang
Nature Methods 16, 143
Research Highlights | 15 November 2018

Personalized DNA methylomics

A study in Nature Medicine reports the DNA methylome and transcriptome of an individual, and suggests that changes in the methylome and transcriptome might be associated with chronic and acute health conditions, respectively.
- Katharine H. Wrighton
Nature Reviews Genetics 20, 4-5
News & Views | 20 July 2018

Infant medulloblastoma — learning new lessons from old strata

Comprehensive molecular characterization of infant medulloblastoma has uncovered the high degree of heterogeneity of this disease. Recent results from the SJYC07 study elegantly reveal that risk stratification can be improved if DNA methylation profiling data are incorporated into clinicopathological criteria. Importantly, the delineation of disease subgroups potentially has major clinical implications.
- Marc Remke
- & Vijay Ramaswamy
Nature Reviews Clinical Oncology 15, 659-660

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Methylation analysis

Latest Research and Reviews

Simultaneous profiling of chromatin accessibility and methylation on human cell lines with nanopore sequencing

Modular affinity-labeling of the cytosine demethylation base elements in DNA

Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study

Structural equation modelling analysis determining causal role among methyltransferases, methylation, and apoptosis during human pregnancy and abortion

DNA methylation of the INSR gene as a mediator of the association between prenatal exposure to famine and adulthood waist circumference

Comprehensive characterization of claudin-low breast tumors reflects the impact of the cell-of-origin on cancer evolution

News and Comment

Closing in on cfDNA-based detection and diagnosis

Reprogrammable CRISPR/dCas9-based recruitment of DNMT1 for site-specific DNA demethylation and gene regulation

Bisulfite-free epigenetic sequencing

Detecting changes in methylation landscape

Personalized DNA methylomics

Infant medulloblastoma — learning new lessons from old strata

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