Methylation analysis

Despite the importance of DNA methylation, accessible and high-throughput methods to profile methylation at the single-cell level are lacking. Here, the authors present sciMETv2, a high-throughput workflow that provides high-quality single-cell methylomes in a robust and simple workflow.

Engineered chromodomains are promising probes to analyze histone methylation. Here the authors designed high-affinity chromodomains for enhanced genome-wide binding analysis, live-cell imaging and ultra-potent CRISPR-based gene repression.

This protocol provides an enzyme-free method to achieve targeted DNA demethylation based on steric hindrance by dCas9 bound at or close to CpG sites of interest to identify DNA methylation marks causal to altered gene expression.

Differences in CpG coverage of targeted bisulfite sequencing methods can be overcome using imputation.