Membrane structure and assembly

  • Article |

    The translocation and assembly module (TAM) is a nanomachine responsible for assembly of proteins into the outer membrane of pathogenic bacteria. Here, Shen et al.use magnetic contrast neutron reflectrometry to visualize movements within the TAM and insertion of a substrate protein into the lipid bilayer.

    • Hsin-Hui Shen
    • , Denisse L. Leyton
    •  & Trevor Lithgow
  • Article |

    Membrane curvature and lipid composition direct the binding of many peripheral membrane proteins. Here, Vanni et al. use a combination of in vitroand molecular dynamics approaches to quantify how lipid-packing defects in membranes of various composition and curvature dictate the membrane adsorption of a model lipid-binding protein.

    • Stefano Vanni
    • , Hisaaki Hirose
    •  & Romain Gautier
  • Article
    | Open Access

    Although many proteins adopt uneven distributions in the plasma membrane, it is not clear how these nanoscale heterogeneities relate to the general protein patterning of the membrane. Saka et al. use click chemistry to reveal the mesoscale organization of membrane proteins into multi-protein assemblies.

    • Sinem K. Saka
    • , Alf Honigmann
    •  & Silvio O. Rizzoli
  • Article
    | Open Access

    There is debate about the structural organization of the yeast centromeric nucleosome and the role of the nonhistone protein Scm3 in its assembly. Dechassaet al.find that yeast centromeric nucleosomes organize DNA in a left-handed superhelix, and show that Scm3 is a specific nucleosome assembly factor.

    • Mekonnen Lemma Dechassa
    • , Katharina Wyns
    •  & Karolin Luger