Article
|
Open Access
Featured
-
-
Article
| Open AccessRegulation of meiotic telomere dynamics through membrane fluidity promoted by AdipoR2-ELOVL2
Meiosis is a specialized cell division for generating germ cells. The authors show that the lipid composition in the cellular membrane influences meiosis-specific chromosomal dynamics in mouse testis.
- Jingjing Zhang
- , Mario Ruiz
- & Hiroki Shibuya
-
Article
| Open AccessRNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis
Gene expression dynamics are tightly regulated during spermatogenesis, with disruptions resulting in infertility. Here they identify a critical role for RNA PolII pausing in spermatogenesis and show that loss of the RNA PolII pausing factor NELF causes meiotic arrest.
- Emily G. Kaye
- , Kavyashree Basavaraju
- & Prabhakara P. Reddi
-
Article
| Open AccessAn oocyte meiotic midbody cap is required for developmental competence in mice
Midbodies form during cell division and play roles in cell function and fate. Here, the authors show that the meiotic midbody in mouse oocytes has a specialized cap structure required to retain nascent proteins in eggs and for full developmental competence.
- Gyu Ik Jung
- , Daniela Londoño-Vásquez
- & Karen Schindler
-
Article
| Open AccessHOP1 and HAP2 are conserved components of the meiosis-related machinery required for successful mating in Leishmania
Genetic exchange has been experimentally demonstrated for Leishmania during sand fly development, indicating a meiotic mechanism. Here the authors show that meiosis-related genes HOP1 and HAP2-2 are essential for Leishmania hybridization in vitro and in sand flies and that their deletion in one or both parents hinders mating competence.
- Carolina Moura Costa Catta-Preta
- , Tiago Rodrigues Ferreira
- & David Sacks
-
Article
| Open AccessFIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination
Assembly and disassembly of RAD51/DMC1 during homologous recombination are tightly regulated. Here, the authors show that the FIGNL1 ATPase limits non-productive assembly of RAD51/DMC1 on single-stranded and double-stranded DNAs during meiosis.
- Masaru Ito
- , Asako Furukohri
- & Akira Shinohara
-
Article
| Open AccessSTRA8–RB interaction is required for timely entry of meiosis in mouse female germ cells
Female germ cells initiate meiosis within a limited time period in the fetal ovary. Here the authors show that the interaction between STRA8 and RB ensures precise timing of meiosis initiation and highlight the regulatory mechanisms underlying female-specific meiotic initiation in mice.
- Ryuki Shimada
- , Yuzuru Kato
- & Kei-ichiro Ishiguro
-
Article
| Open AccessSCFRMF mediates degradation of the meiosis-specific recombinase DMC1
In eukaryotes the recombinase DMC1 is required for meiotic recombination, but it remains unclear how DMC1 stability is regulated. Here the authors identify a SCF complex which mediates ubiquitination and proteasomal degradation of DMC1.
- Wanyue Xu
- , Yue Yu
- & Yingxiang Wang
-
Article
| Open AccessKinetochore component function in C. elegans oocytes revealed by 4D tracking of holocentric chromosomes
The exact function of kinetochore proteins in meiosis remains unclear. Using live imaging of C. elegans oocytes, the authors systematically study the contribution of each kinetochore sub-complex and describe a push-pull mechanism that confers robustness to chromosome segregation.
- Laras Pitayu-Nugroho
- , Mélanie Aubry
- & Julien Dumont
-
Article
| Open AccessDistinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity
Male meiosis relies on canonical centrosomes for spindle formation, but how this differs from acentrosomal oocyte meiosis is unclear. Here they show that spindle formation in sperm relies on DYNLRB2, similar to the activity of DYNLRB1 in mitotic cells.
- Shuwen He
- , John P. Gillies
- & Hiroki Shibuya
-
Article
| Open AccessPRC1-mediated epigenetic programming is required to generate the ovarian reserve
In humans, the ovarian reserve is maintained over decades by meiotic arrest of oocytes. Here the authors show that Polycomb Repressive Complex 1 (PRC1)-mediated epigenetic programming is essential for formation of ovarian reserve and thus female reproductive lifespan.
- Mengwen Hu
- , Yu-Han Yeh
- & Satoshi H. Namekawa
-
Article
| Open AccessFANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis
The FANCM helicase functions in limiting crossovers (COs) by unwinding inter-homolog repair intermediates. Here, the authors generate null mutants of fancm in tetraploid and hexaploid wheat and show that FANCM promotes class I interfering COs and suppresses class II noninterfering COs in wheat meiosis.
- Stuart D. Desjardins
- , James Simmonds
- & James D. Higgins
-
Article
| Open Access3D chromatin remodelling in the germ line modulates genome evolutionary plasticity
The role of genome folding in the heritability and evolvability of structural variations is not well understood. Here the authors investigate the impact of the three-dimensional genome topology of germ cells in the formation and transmission of gross structural genomic changes detected from comparing whole-genome sequences of 14 rodent species.
- Lucía Álvarez-González
- , Frances Burden
- & Aurora Ruiz-Herrera
-
Article
| Open AccessNitrogen nutrition contributes to plant fertility by affecting meiosis initiation
Nitrogen deficiency can cause floral abortion during reproductive development of rice. Here the authors show that when nitrogen is limited, rice plants require the ETFβ protein, which is involved in branched chain amino acid catabolism, to promote nitrogen reutilization and support the initiation of meiosis.
- Han Yang
- , Yafei Li
- & Zhukuan Cheng
-
Article
| Open AccessMammalian SWI/SNF chromatin remodeler is essential for reductional meiosis in males
The mammalian SWI/SNF nucleosome remodeler is required for spermatogenesis. Here, the authors show that PBAF is essential for meiotic cell division in males and required to activate the expression of critical genes involved in spindle assembly and nuclear division in spermatocytes.
- Debashish U. Menon
- , Oleksandr Kirsanov
- & Terry Magnuson
-
Article
| Open AccessCENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes
Chromosome segregation is essential to avoid aneuploidy, yet in mammalian oocytes it progressively fails in an age-dependent manner. Here the authors identify CENP-V as a microtubule binding and bundling protein crucial to faithful oocyte meiosis, and present Cenp-V−/− oocytes as revealing age-dependent weakening of the spindle assembly checkpoint.
- Dalileh Nabi
- , Hauke Drechsler
- & Mariola Chacón
-
Article
| Open AccessStage-resolved Hi-C analyses reveal meiotic chromosome organizational features influencing homolog alignment
During meiosis, chromosomes undergo dramatic changes in morphology and intranuclear positioning. Here the authors mapped the 3D genome architecture throughout mouse spermatogenesis by Hi-C of sorted cells to reveal the contributions of transcriptional activity and mechanical force in modulating homolog alignment and recombination.
- Wu Zuo
- , Guangming Chen
- & Qian Bian
-
Article
| Open AccessA prometaphase mechanism of securin destruction is essential for meiotic progression in mouse oocytes
Securin inhibits the protease separase and must be removed before anaphase to ensure timely chromosome segregation. Here, the authors define a mechanism of securin destruction in prometaphase I in mouse oocytes and demonstrate its importance for successful meiotic progression.
- Christopher Thomas
- , Benjamin Wetherall
- & Suzanne Madgwick
-
Article
| Open AccessThe study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals an Arpp19/PP2A-B55 feedback loop
Progression of the cell division cycle requires feedback loops including those of phosphorylation and dephosphorylation; however the precise regulation of phosphorylation kinetics of Arpp19, an inhibitor of protein phosphatase 2A, is unclear. Here, the authors report that feedback between phosphorylation states of Ser67 and Ser109 of Arpp19 coordinates Arpp19-dependent inhibition of PP2A-B55 and Cyclin B activation during cell cycle progression.
- Jean Claude Labbé
- , Suzanne Vigneron
- & Thierry Lorca
-
Article
| Open AccessMeiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis
The authors add to our knowledge of the transcriptional regulation of the meiotic program in mice spermatocytes, showing ZFP541 regulates meiotic prophase and transition to the division phase by being the target for upstream factors MEIOSIN/STRA8.
- Yuki Horisawa-Takada
- , Chisato Kodera
- & Kei-Ichiro Ishiguro
-
Article
| Open AccessThe M-phase regulatory phosphatase PP2A-B55δ opposes protein kinase A on Arpp19 to initiate meiotic division
Mechanisms triggering meiotic divisions of oocytes remain unclear. Here, the authors report that meiosis resumption relies on the timely phosphorylation of Arpp19 protein at two distinct sites, which depends on two kinases (PKA and Gwl) and a single phosphatase (PP2A-B55δ).
- Tom Lemonnier
- , Enrico Maria Daldello
- & Aude Dupré
-
Article
| Open AccessNutrient restriction synergizes with retinoic acid to induce mammalian meiotic initiation in vitro
Retinoic acid is necessary but not sufficient to induce meiosis. Here, the authors use primary mouse undifferentiated spermatogonia culture to show that nutrient restriction, an inducer of yeast meiosis, combined with retinoic acid induces meiotic gene and chromosome programs in mammalian germ cells.
- Xiaoyu Zhang
- , Sumedha Gunewardena
- & Ning Wang
-
Article
| Open AccessSurveillance of cohesin-supported chromosome structure controls meiotic progression
Meiosis-specific cohesins and the synaptonemal complex are essential for meiotic chromosome structure and function. Here the authors show that continued surveillance of these chromosome structures controls meiotic progression by regulating CHK-2, a master regulator of pairing and recombination.
- Maikel Castellano-Pozo
- , Sarai Pacheco
- & Enrique Martinez-Perez
-
Article
| Open AccessNampt-mediated spindle sizing secures a post-anaphase increase in spindle speed required for extreme asymmetry
Meiotic cell division in oocytes is asymmetric and requires microtubule spindle migration after anaphase-onset. Here, the authors show that Nampt, an enzyme of the Nicotinamide adenine dinucleotide (NAD) biosynthetic pathway, contributes to post-anaphase spindle migration and oocyte division asymmetry by controlling spindle length.
- Zhe Wei
- , Jessica Greaney
- & Hayden Anthony Homer
-
Article
| Open AccessProline-rich protein PRR19 functions with cyclin-like CNTD1 to promote meiotic crossing over in mouse
Crossing over is a critical process during meiosis, although the regulation of this process still remains somewhat elusive. Here, the authors show that PRR19 partners with CNTD1 to enable formation of crossover-specific recombination complexes in mouse germ cells.
- Anastasiia Bondarieva
- , Kavya Raveendran
- & Attila Tóth
-
Article
| Open AccessPrc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes
Oocyte meiosis must achieve spindle bipolarization without predefined spatial cues. Yoshida et al. demonstrate that spindle bipolarization during meiosis I in mouse oocytes requires kinetochores to prevent chromosome segregation errors, a phenomenon that does not occur in error-prone human oocytes.
- Shuhei Yoshida
- , Sui Nishiyama
- & Tomoya S. Kitajima
-
Article
| Open AccessRegulated repression governs the cell fate promoter controlling yeast meiosis
In budding yeast, the decision to enter meiosis is defined by nutrient and mating-type signals regulating expression of the master transcription factor for meiotic entry, IME1. Here, the authors characterize the steps involved in regulating the entering in meiosis via the co-repressor complex Tup1-Cyc8.
- Janis Tam
- & Folkert J. van Werven
-
Article
| Open AccessThe BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells
In meiosis, BRCA2 associates to MEILB2 localising at DNA double-strand breaks (DSBs). Here, the authors identify BRCA2 and MEILB2- associating protein 1 termed BRME1 to work together in regulating meiotic recombination.
- Jingjing Zhang
- , Manickam Gurusaran
- & Hiroki Shibuya
-
Article
| Open AccessArtificially decreasing cortical tension generates aneuploidy in mouse oocytes
The developmental potential of human and murine oocytes is predicted by their mechanical properties. Here the authors show that artificial reduction of cortex tension produces aneuploid mouse oocytes and speculate that this may contribute to the high aneuploidy rate typical of female meiosis.
- Isma Bennabi
- , Flora Crozet
- & Marie-Emilie Terret
-
Article
| Open AccessDynamic organelle distribution initiates actin-based spindle migration in mouse oocytes
Mammalian oocytes divide asymmetrically during meiotic maturation. Here, the authors show that spindle movement away from oocyte center depends on actin filaments nucleated from the spindle periphery pushing against surrounding mitochondria, which polarizes spontaneously to produce directional spindle motion.
- Xing Duan
- , Yizeng Li
- & Rong Li
-
Article
| Open AccessActin-microtubule interplay coordinates spindle assembly in human oocytes
Actin and microtubules contribute to successful oocyte maturation, but if and how these two networks communicate in human oocytes is unclear. Here the authors show that actin-microtubule interactions are essential for correct segregation of human nuclear genomic content.
- Johannes Roeles
- & Georgios Tsiavaliaris
-
Article
| Open AccessGenome-wide recombination map construction from single individuals using linked-read sequencing
Variation of recombination rates within genomes has important implications in genetics and evolution. Here, the authors develop a method for building genome-wide recombination maps from single individuals using linked-read sequencing data, and report its application in mouse and stickleback fish.
- Andreea Dréau
- , Vrinda Venu
- & Felicity C. Jones
-
Article
| Open Accessm6A modification of a 3′ UTR site reduces RME1 mRNA levels to promote meiosis
Ime4p is a yeast N6-methyladenosine (m6A) methyltransferase with an unknown role in meiosis. Rme1p is a repressor of meiosis. Here the authors show that Ime4p methylates RME1 3′ UTR to reduce its expression and enable meiosis, thus providing an example of an m6A site with a physiological role.
- G. Guy Bushkin
- , David Pincus
- & Gerald R. Fink
-
Article
| Open AccessReducing MSH4 copy number prevents meiotic crossovers between non-homologous chromosomes in Brassica napus
Non-homologous crossovers impair correct chromosome segregation in allopolyploids. Here the authors show that most non-homologous crossovers in Brassica napus arise from MSH4-dependent recombination and provide evidence that post-polyploidization reduction of MSH4 duplicate stabilizes meiosis.
- Adrián Gonzalo
- , Marie-Odile Lucas
- & Eric Jenczewski
-
Article
| Open AccessA meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination
Homology directed repair of meiotic double-strand breaks functions via recruitment and assembly of strand-exchange proteins called recombinases. Here the authors reveal and characterize a BRCA2 interactor regulating meiotic recombinases that localizes to chromosomal axes and facilitates the repair of meiotic DSBs.
- Jingjing Zhang
- , Yasuhiro Fujiwara
- & Hiroki Shibuya
-
Article
| Open AccessThe meiotic TERB1-TERB2-MAJIN complex tethers telomeres to the nuclear envelope
The TERB1-TERB2-MAJIN complex mediates the attachment of telomeres to the nuclear envelope. Here the authors present the crystal structures of the human TERB1-TERB2 and TERB2-MAJIN subcomplexes and show that Terb2 mutations, which abolish complex formation cause aberrant homologous pairing and disordered synapsis in mouse.
- Yan Wang
- , Yanyan Chen
- & Ming Lei
-
Article
| Open AccessStructural basis of meiotic telomere attachment to the nuclear envelope by MAJIN-TERB2-TERB1
The meiotic telomere complex (MAJIN, TERB1, TERB2) tethers telomere ends to the nuclear envelope. Here the authors present the crystal structure of human MAJIN-TERB2 and combine biophysical approaches and structured illumination microscopy analysis of mouse meiotic chromosomes to characterize the molecular architecture of the wider MAJIN-TERB2-TERB1 complex and its interactions with TRF1.
- James M. Dunce
- , Amy E. Milburn
- & Owen R. Davies
-
Article
| Open AccessShu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination
Homologous recombination ensures genome integrity during meiotic recombination. Here the authors reveal that factors SWS1 and SWSAP1 are critical for meiotic homologues recombination, particularly in promoting assembly of RAD51 and DMC1 on early recombination intermediates.
- Carla M. Abreu
- , Rohit Prakash
- & Maria Jasin
-
Article
| Open AccessCdk1 inactivation induces post-anaphase-onset spindle migration and membrane protrusion required for extreme asymmetry in mouse oocytes
Female meiotic divisions are asymmetric with the formation of large oocytes and small polar bodies, thought to result from cortical spindle placement before anaphase. The authors find that Cdk1 inactivation triggers F-actin dependent post-anaphase spindle migration, resulting in cortical protrusion.
- Zhe Wei
- , Jessica Greaney
- & Hayden A. Homer
-
Article
| Open AccessATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis
The histone H3 CENP-A is known to play a role during meiosis but its role in the testes in the fly is unknown. Here, the authors identify the mitochondrial metabolic protein complex ATP synthase F1 as interacting with CENP-A, promoting centromere cohesion during meiosis and affecting fly fertility.
- Caitríona M. Collins
- , Beatrice Malacrida
- & Elaine M. Dunleavy
-
Article
| Open AccessATR is a multifunctional regulator of male mouse meiosis
ATR kinase is required for meiosis in non-mammalian model organisms. Here the authors demonstrate, using a tissue-specific knockout approach, that ATR is also essential for male meiosis in mouse, regulating meiotic recombination and synapsis.
- Alexander Widger
- , Shantha K. Mahadevaiah
- & James M.A. Turner
-
Article
| Open AccessATR is required to complete meiotic recombination in mice
ATR kinase is required for meiosis in non-mammalian model organisms. Here the authors demonstrate, using a hypomorphic Atr mutation and chemical inhibition, that ATR is also essential for male meiosis in mouse, regulating meiotic recombination and synapsis.
- Sarai Pacheco
- , Andros Maldonado-Linares
- & Ignasi Roig
-
Article
| Open AccessSpatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis
During meiosis, step-wise release of sister chromatid cohesion mediated by REC-8 cohesin is required for the formation of haploid gametes. Here, the authors show that in C. elegans oocytes, regulated recruitment of Aurora B kinase ensures the correct distribution of REC-8 phosphorylation, which promotes cohesion release.
- Nuria Ferrandiz
- , Consuelo Barroso
- & Enrique Martinez-Perez
-
Article
| Open AccessChromosome segregation occurs by microtubule pushing in oocytes
In oocytes of most species atypical spindles assembled in the absence of centrosomes drive chromosome segregation, however the forces driving this process are unclear. Here the authors found that spindle poles are largely dispensable and that inter-chromosomal microtubules of the central spindle control chromosomal segregation.
- Kimberley Laband
- , Rémi Le Borgne
- & Julien Dumont
-
Article
| Open AccessA cdk1 gradient guides surface contraction waves in oocytes
Surface contraction waves (SCWs) are prominent shape changes coupled to cell cycle transitions in oocytes. Here the authors show that SCWs are patterned by the spatiotemporal integration of two conserved modules, cdk1-cyclinB for cell cycle regulation and RhoA/Rok/NMYII for actomyosin contractility.
- Johanna Bischof
- , Christoph A. Brand
- & Péter Lénárt
-
Article
| Open AccessMps1 kinase-dependent Sgo2 centromere localisation mediates cohesin protection in mouse oocyte meiosis I
In meiosis I centromeric cohesin is protected by Sgo2 from Separase-mediated cleavage ensuring that sister chromatids are kept together until their separation in meiosis II. Here the authors demonstrate that Bub1 and Mps1 kinase activities are required for Sgo2 localisation to the centromere region.
- Warif El Yakoubi
- , Eulalie Buffin
- & Katja Wassmann
-
Article
| Open AccessMaternal age-dependent APC/C-mediated decrease in securin causes premature sister chromatid separation in meiosis II
Sister chromatid cohesion during meiosis II (MII), maintained by securin-mediated inhibition of separase, is reduced in aged mouse oocytes. Here the authors show that, in MII oocytes, securin levels are reduced by increased destruction by the anaphase promoting complex/cyclosome.
- Ibtissem Nabti
- , Rosanna Grimes
- & John Carroll
-
Article
| Open AccessC14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility
The synaptonemal complex is a meiosis-specific proteinaceous structure that supports homologous chromosome pairs during meiosis. Here, the authors show that SIX6OS1 (of previously unknown function) is part of the synaptonemal complex central element and upon deletion in mice, causes defective chromosome synapsis and infertility.
- Laura Gómez-H
- , Natalia Felipe-Medina
- & Alberto M. Pendas
-
Article
| Open AccessEarly programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling
Meiotically arrested oocytes retain transcriptional ability despite chromosome condensation. Here, the authors show that Drosophilaoocytes regulate meiotic transcription and chromatin remodelling through the programming, in early oogenesis, of an extremely diversified epigenome.
- Paulo Navarro-Costa
- , Alicia McCarthy
- & Rui G. Martinho
-
Article
| Open AccessEssential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
CDKs play central roles in cell cycle regulation and are normally activated by cyclins. Here the authors show that RingoA induces a cyclin-independent function of CDK2 at meiotic telomeres, which regulates their tethering to the nuclear envelope and proper synapsis of homologous chromosomes.
- Petra Mikolcevic
- , Michitaka Isoda
- & Angel R. Nebreda