Article
|
Open Access
Featured
-
-
Article
| Open Access
Programmable design of isothermal nucleic acid diagnostic assays through abstraction-based models
Detecting nucleic acids often requires choosing between different amplification mechanisms. Here the authors present a generalisable and programmable isothermal methodology, demonstrated in clinical applications, including for multiplexed detection of short miRNAs.
- Gaolian Xu
- , Julien Reboud
- & Jonathan M. Cooper
-
Article
| Open Access
Finger sweat analysis enables short interval metabolic biomonitoring in humans
Biomonitoring of sweat from fingertips overcomes current limitations in time-resolved metabolomic profiling of humans and may prove to become a powerful, noninvasive tool for precision medicine. Here, in a feasibility study of short interval sampling of sweat from fingertips, the authors assay individual dynamic metabolic patterns of endogenous and exogenous molecules.
- Julia Brunmair
- , Mathias Gotsmy
- & Christopher Gerner
-
Article
| Open Access
Metabolomics of sebum reveals lipid dysregulation in Parkinson’s disease
Studies of metabolites in neurodegeneration have not yet used sebum as a source fluid. Here the authors demonstrate the potential of metabolomics of sebum samples from individuals with Parkinson’s disease and controls.
- Eleanor Sinclair
- , Drupad K. Trivedi
- & Perdita Barran
-
Article
| Open Access
Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
The detection of pathogens in food and clinical samples remains a challenge. Here, Shen et al. present a detection system, involving a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components, that can detect very low numbers of a bacterial pathogen in milk and serum samples without isolation.
- Jinjin Shen
- , Xiaoming Zhou
- & Da Xing
-
Article
| Open Access
Simultaneous multi-signal quantification for highly precise serodiagnosis utilizing a rationally constructed platform
Single channel detection methods often suffer from false positives when analysing biological samples. Here, the authors report on the development of a three-channel detection device for measuring hydrogen sulphide in serum and demonstrate application in an in vivo model.
- Yuxin Liu
- , Zheng Wei
- & Zhanfang Ma
-
Article
| Open Access
Highly selective detection of methanol over ethanol by a handheld gas sensor
Methanol poisoning is frequent and dangerous, but selective sensors able to work in the presence of an ethanol background are missing. Here the authors propose an easy to operate sensor incorporating a separation column, able to sense toxic methanol levels in alcoholic beverages and human breath.
- J. van den Broek
- , S. Abegg
- & A. T. Güntner
-
Review Article
| Open Access
Ligand design strategies to increase stability of gadolinium-based magnetic resonance imaging contrast agents
Gadolinium(III) complexes are strong enhancers of magnetic resonance imaging (MRI) signals, thus are widely used as contrast agents despite their potential toxicity. Here, the authors review ligand design approaches aimed at improving the stability of Gd(III)-based MRI contrast agents.
- Thomas J. Clough
- , Lijun Jiang
- & Nicholas J. Long
-
Article
| Open Access
Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection
Early detection of blood stream infections is essential for providing effective treatments. Here the authors present a system integrating DNAzyme sensors, droplet microfluidics and a high-throughput 3D particle counter that can detect specific, single bacterial cells in blood within a few hours.
- Dong-Ku Kang
- , M. Monsur Ali
- & Weian Zhao
Rapid and visual identification of β-lactamase subtypes for precision antibiotic therapy