Logic gates articles from across Nature Portfolio

Transcription factor over-expression-based cellular conversion methods often endure low conversion efficiency. Here the authors show how to increase conversion efficiency by combining a computational method for prioritizing more efficient TF combinations with a transposon-based genomic integration system for delivery.

Genetic circuits can be engineered to generate predefined outcomes, however host context is a crucial factor in performance. Here the authors characterise twenty NOT gates in seven different bacteria to understand and predict interoperability and portability across hosts.

This study describes design automation and predictable gene regulatory network engineering in a eukaryotic microorganism.

The availability of high performance recombinases with low basal activity and high dynamic range is limited. Here the authors present a library of over 20 orthogonal split recombinases that can be induced by small molecules, light and temperature in vivo.