Light-sheet microscopy

Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation compared to conventional light microscopy methods.

Latest Research and Reviews

  • Research
    | Open Access

    It has been challenging to perform super-resolution imaging in large volumes due to aberrations encountered. Here, the authors combine single-wavelength Bessel lightsheet localization microscopy with tissue clearing techniques and image neurons across the whole brain of adult fruit flies.

    • Li-An Chu
    • , Chieh-Han Lu
    • , Shun-Min Yang
    • , Yen-Ting Liu
    • , Kuan-Lin Feng
    • , Yun-Chi Tsai
    • , Wei-Kun Chang
    • , Wen-Cheng Wang
    • , Shu-Wei Chang
    • , Peilin Chen
    • , Ting-Kuo Lee
    • , Yeu-Kuang Hwu
    • , Ann-Shyn Chiang
    •  & Bi-Chang Chen
  • Research
    | Open Access

    Light sheet microscopy holds potential for imaging dynamics in 3D biological specimens, but is limited by scan speed and camera acquisition rate. Here the authors address both issues by developing speed-optimized Objective Coupled Planar Illumination and parallelizing image acquisition across cameras to achieve 40 Hz imaging over thick samples.

    • Cody J. Greer
    •  & Timothy E. Holy
  • Research |

    SCAPE 2.0 is a versatile imaging platform that enables real-time three-dimensional microscopy of cellular function and dynamic motion in living organisms at over 100 volumes per second with minimal photodamage, and high-throughput structural imaging in fixed, cleared and expanded samples.

    • Venkatakaushik Voleti
    • , Kripa B. Patel
    • , Wenze Li
    • , Citlali Perez Campos
    • , Srinidhi Bharadwaj
    • , Hang Yu
    • , Caitlin Ford
    • , Malte J. Casper
    • , Richard Wenwei Yan
    • , Wenxuan Liang
    • , Chentao Wen
    • , Koutarou D. Kimura
    • , Kimara L. Targoff
    •  & Elizabeth M. C. Hillman
    Nature Methods 16, 1054-1062
  • Research |

    The mesoSPIM is an open hardware axially scanned light-sheet microscope for the rapid imaging of large cleared samples with isotropic resolution.

    • Fabian F. Voigt
    • , Daniel Kirschenbaum
    • , Evgenia Platonova
    • , Stéphane Pagès
    • , Robert A. A. Campbell
    • , Rahel Kastli
    • , Martina Schaettin
    • , Ladan Egolf
    • , Alexander van der Bourg
    • , Philipp Bethge
    • , Karen Haenraets
    • , Noémie Frézel
    • , Thomas Topilko
    • , Paola Perin
    • , Daniel Hillier
    • , Sven Hildebrand
    • , Anna Schueth
    • , Alard Roebroeck
    • , Botond Roska
    • , Esther T. Stoeckli
    • , Roberto Pizzala
    • , Nicolas Renier
    • , Hanns Ulrich Zeilhofer
    • , Theofanis Karayannis
    • , Urs Ziegler
    • , Laura Batti
    • , Anthony Holtmaat
    • , Christian Lüscher
    • , Adriano Aguzzi
    •  & Fritjof Helmchen
    Nature Methods 16, 1105-1108

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